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Titolo:
THE SEPARATION OF GREEN TEA CATECHINS BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY
Autore:
NELSON BC; THOMAS JB; WISE SA; DALLUGE JJ;
Indirizzi:
NIST,CHEM SCI & TECHNOL LAB,DIV ANALYT CHEM GAITHERSBURG MD 20899 NIST,CHEM SCI & TECHNOL LAB,DIV ANALYT CHEM GAITHERSBURG MD 20899
Titolo Testata:
The Journal of microcolumn separations
fascicolo: 8, volume: 10, anno: 1998,
pagine: 671 - 679
SICI:
1040-7685(1998)10:8<671:TSOGTC>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; (-)-EPIGALLOCATECHIN GALLATE; CAPILLARY ELECTROPHORESIS; INDIVIDUAL CATECHINS; GAS-CHROMATOGRAPHY; MASS-SPECTROMETRY; TUMOR INITIATION; SENCAR MICE; POLYPHENOLS; SKIN;
Keywords:
CAPILLARY ELECTROPHORESIS; CATECHINS; CYCLODEXTRIN MODIFIER; GREEN TEA; MICELLAR ELECTROKINETIC CHROMATOGRAPHY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
B.C. Nelson et al., "THE SEPARATION OF GREEN TEA CATECHINS BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY", The Journal of microcolumn separations, 10(8), 1998, pp. 671-679

Abstract

A micellar electrokinetic chromatographic (MEKC) separation system was developed for the efficient separation of biologically and clinically important green tea catechins. Six structurally isomeric tea catechins, e.g., (-)epigallocatechin-3-gallate (EGCG), (-)epicatechin gallate(ECG), (-)gallocatechin gallate (GCG), (-)epigallocatechin (EGC), (-)epicatechin (EC), and (+)catechin (C), were effectively separated using a tetraborate/sodium dodecyl sulfate (SDS)/beta-cyclodextrin micellar buffer system. The effects of several critical separation parameters(micelle charge type, surfactant type, organic solvent buffer modifier, micelle concentration, buffer pH, and cyclodextrin buffer modifier)were evaluated for enhanced isomer separation and resolution. The pH was shown to be a strong factor influencing the separation of the isomers, but baseline resolution of all six structural isomers was only effected when a micellar buffer system contained P-cyclodextrin buffer modifier. The proficiency of the developed method was tested by determining the analytical levels (mg/g) of crude catechins in aqueous extractions of three varieties of green tea (Camellia sinesis). (C) 1998 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 11:05:02