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Titolo:
USE OF PRINS TECHNIQUE FOR CHROMOSOMAL ANALYSIS OF FETAL CELLS IN MATERNAL BLOOD
Autore:
ORSETTI B; LEFORT G; BOULOT P; ANDREO B; PELLESTOR F;
Indirizzi:
CNRS,IGH,UPR 1142,141 RUE CARDONILLE F-34396 MONTPELLIER FRANCE HOP ARNAUD DE VILLENEUVE,LAB CYTOGENET F-34033 MONTPELLIER FRANCE HOP ARNAUD DE VILLENEUVE,SERV FOETOPATHOL F-34033 MONTPELLIER FRANCE
Titolo Testata:
Annales de pathologie
fascicolo: 5, volume: 18, anno: 1998,
pagine: 377 - 384
SICI:
0242-6498(1998)18:5<377:UOPTFC>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
FRE
Soggetto:
ALPHA-SATELLITE DNA; PRENATAL-DIAGNOSIS; NUCLEATED ERYTHROCYTES; PERIPHERAL-BLOOD; INSITU HYBRIDIZATION; PREGNANT-WOMEN; VENOUS-BLOOD; THALASSEMIA; CIRCULATION; TRISOMY-21;
Keywords:
FETAL CELLS; MATERNAL BLOOD; HISTOPAQUE DOUBLE DENSITY GRADIENT; PRINS; CHROMOSOMES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
B. Orsetti et al., "USE OF PRINS TECHNIQUE FOR CHROMOSOMAL ANALYSIS OF FETAL CELLS IN MATERNAL BLOOD", Annales de pathologie, 18(5), 1998, pp. 377-384

Abstract

Both detection and chromosomal analysis of fetal cells present ill the maternal circulation can be performed rising Histopaque double density gradient centrifugation? followed by? primed in situ (PRINS) labeling technique. This approach has been tested on blood samples from 15 pregnant women and 6 control donors with primers specific for chp chromosomes 9, X and Y. The cell separation technique allows recovery Of both mononuclear cells and polynuclear cells with a 97% efficiency. PRINS labeling Iz,as successful in 100% cells from control blood samples. Among patient samples, 2 ''false-negative'' results were observed. These preliminary results suggest that the present protocol might DE efficient for non-invasive prenatal chromosome analysis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:54:28