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Titolo:
EVIDENCE FOR 2 DIFFERENT TYPES OF P2 RECEPTORS STIMULATING INSULIN-SECRETION FROM PANCREATIC B-CELL
Autore:
PETIT P; HILLAIREBUYS D; MANTEGHETTI M; DEBRUS S; CHAPAL J; LOUBATIERESMARIANI MM;
Indirizzi:
UNIV MONTPELLIER 1,INST BIOL,PHARMACOL LAB,FAC MED,UPRES EA 1677,4 BLVD HENRI IV F-34060 MONTPELLIER 1 FRANCE
Titolo Testata:
British Journal of Pharmacology
fascicolo: 6, volume: 125, anno: 1998,
pagine: 1368 - 1374
SICI:
0007-1188(1998)125:6<1368:EF2DTO>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFUSED RAT PANCREAS; ADENINE-NUCLEOTIDE ANALOGS; BETA-CELLS; PURINERGIC RECEPTORS; EXTRACELLULAR ATP; VASCULAR BED; ISLET CELLS; DIAZOXIDE; GLUCOSE; P2-PURINOCEPTOR;
Keywords:
P2 RECEPTORS; INSULIN SECRETION; RUBIDIUM EFFLUX; ALPHA,BETA-METHYLENE ATP; ADP-BETA-S; DESENSITIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
P. Petit et al., "EVIDENCE FOR 2 DIFFERENT TYPES OF P2 RECEPTORS STIMULATING INSULIN-SECRETION FROM PANCREATIC B-CELL", British Journal of Pharmacology, 125(6), 1998, pp. 1368-1374

Abstract

1 Adenine nucleotides have been shown to stimulate insulin secretion by acting on P2 receptors of the P2Y type. Since there have been some discrepancies in the insulin response of different analogues of ATP and ADP, we investigated whether two different types of P2 receptors exist on pancreatic B cells. The effects of alpha,beta-methylene ATP, which is more specific for the P2X subtype, were studied in vitro in pancreatic islets and isolated perfused pancreas from rats, in comparison with the potent P2Y receptor agonist ADP beta S. 2 In isolated islets,incubated with a slightly stimulating glucose concentration (8.3 mM),alpha,beta-me ATP (200 mu M) and ADP beta S (50 mu M) similarly stimulated insulin secretion; by contrast, under a non stimulating glucose concentration (8.3 mM), alpha,beta-me ATP was still effective whereas ADP beta S was not. In the same way, in islets perifused with 3 mM glucose, alpha,beta-me ATP but not ADP beta S induced a partial but significant reduction in the peak Rb-86 efflux induced by the ATP-dependentpotassium channel opener diazoxide. 3 In the isolated pancreas, perfused with a non stimulating glucose concentration (4.2 mM), ADP beta S and alpha,beta-me ATP (5-50 mu M), administered for 10 min, induced animmediate, transient and concentration-dependent increase in the insulin secretion; their relative potency was not significantly different. In contrast, with a slightly stimulating glucose concentration (8.3 mM), ADP beta S was previously shown to be 100 fold more potent than alpha,beta-me ATP. Furthermore, at 4.2 mM glucose a second administration of alpha,beta-me ATP was ineffective. In the same way, ADP beta S was also able to desensitize its own insulin response. At 3 mM glucose, alpha,beta-me ATP as well as ADP beta S (50 mu M) induced a transient stimulation of insulin secretion and down regulated the action of eachother. 4 These results give evidence that pancreatic B cells, in addition to P2Y receptors, which potentiate glucose-induced insulin secretion, are provided with P2X receptors, which transiently stimulate insulin release at low non-stimulating glucose concentration and slightly affect the potassium conductance of the membrane.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 15:33:40