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Titolo:
THE PRESENCE OF 2 DISTINCT 8-OXOGUANINE REPAIR ENZYMES IN HUMAN-CELLS- THEIR POTENTIAL COMPLEMENTARY ROLES IN PREVENTING MUTATION
Autore:
HAZRA IK; IZUMI T; MAIDT L; FLOYD RA; MITRA S;
Indirizzi:
UNIV TEXAS,MED BRANCH,SEALY CTR MOL SCI,6-136 MED RES BLDG,ROUTE 1079GALVESTON TX 77555 UNIV TEXAS,MED BRANCH,SEALY CTR MOL SCI GALVESTON TX 77555 UNIV TEXAS,MED BRANCH,DEPT HUMAN BIOL CHEM & GENET GALVESTON TX 77555 OKLAHOMA MED RES FDN OKLAHOMA CITY OK 73104
Titolo Testata:
Nucleic acids research
fascicolo: 22, volume: 26, anno: 1998,
pagine: 5116 - 5122
SICI:
0305-1048(1998)26:22<5116:TPO2D8>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
OXIDATIVE DNA-DAMAGE; ESCHERICHIA-COLI; SACCHAROMYCES-CEREVISIAE; OGG1 GENE; 8-HYDROXYGUANINE 7,8-DIHYDRO-8-OXOGUANINE; EXCISION-REPAIR; MISMATCH REPAIR; HUMAN HOMOLOG; FPG PROTEIN; GLYCOSYLASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
I.K. Hazra et al., "THE PRESENCE OF 2 DISTINCT 8-OXOGUANINE REPAIR ENZYMES IN HUMAN-CELLS- THEIR POTENTIAL COMPLEMENTARY ROLES IN PREVENTING MUTATION", Nucleic acids research, 26(22), 1998, pp. 5116-5122

Abstract

8-Oxoguanine (8-oxoG), induced by reactive oxygen species (ROS) and ionizing radiation, is arguably the most important mutagenic lesion in DNA, This oxidized base, because of its mispairing with A, induces GC-->TA transversion mutations often observed spontaneously in tumor cells. The human cDNA encoding the repair enzyme 8-oxoG-DNA glycosylase (OGG-1) has recently been cloned, however, its activity was never detected in cells, Here we show that the apparent lack of this activity could be due to the presence of an 8-oxoG-specific DNA binding protein. Moreover, we demonstrate the presence of two antigenically distinct OGG activities with an identical reaction mechanism in human cell (HeLa) extracts, The 38 kDa OGG-1, identical to the cloned enzyme, cleaves 8-oxoG when paired with cytosine, thymine and guanine but not adenine in DNA, In contrast, the newly discovered 36 kDa OGG-2 prefers 8-oxoG paired with G and A, We propose that OGG-1 and OGG-2 have distinct antimutagenic functions in vivo. OGG-1 prevents mutation by removing 8-oxoG formed in DNA in situ and paired with C, while OGG-2 removes 8-oxoG that is incorporated opposite A in DNA from ROS-induced 8-oxodGTP, We predict that OGG-2 specifically removes such 8-oxoG residues only from the nascent strand, possibly by utilizing the same mechanism as the DNAmismatch repair pathway.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 10:47:50