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Titolo:
A UV RESONANCE RAMAN INVESTIGATION OF POLY(RL) - EVIDENCE FOR CATION-DEPENDENT STRUCTURAL PERTURBATIONS
Autore:
MUKERJI I; SOKOLOV L; MIHAILESCU MR;
Indirizzi:
WESLEYAN UNIV,DEPT MOL BIOL & BIOCHEM,MOL BIOPHYS PROGRAM MIDDLETOWN CT 06459
Titolo Testata:
Biopolymers
fascicolo: 7, volume: 46, anno: 1998,
pagine: 475 - 487
SICI:
0006-3525(1998)46:7<475:AURRIO>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
TELOMERE-BINDING PROTEIN; G-QUARTET FORMATION; POLYINOSINIC ACID; POLYGUANYLIC ACID; CRYSTAL-STRUCTURE; DNA QUADRUPLEXES; AQUEOUS-SOLUTION; VIBRATIONAL ANALYSIS; SELF-ASSOCIATION; BETA-SUBUNIT;
Keywords:
POLY(RL); UV RESONANCE RAMAN SPECTROSCOPY; CATION-DEPENDENT STRUCTURAL PERTURBATIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
I. Mukerji et al., "A UV RESONANCE RAMAN INVESTIGATION OF POLY(RL) - EVIDENCE FOR CATION-DEPENDENT STRUCTURAL PERTURBATIONS", Biopolymers, 46(7), 1998, pp. 475-487

Abstract

Poly(rI) stabilized by either Na+ or K+ was investigated using uv resonance Raman (UVRR) spectroscopy. Raman excitation profiles of inosine5'-monophosphate demonstrated the 250 nm excitation selectively enhances base stacking interactions, while ribose and carbonyl stretching vibrations are preferentially enhanced with 210 nm excitation. These wavelengths were used to examine the structure of poly(rI) in the presence of either K+ or Na+ as a function of temperature. temperature. UVRRstudies revealed that the K+ stabilized form is more thermally stable, yielding a T-m of similar to 47 degrees C compared to a T-m of similar to 30 degrees C for the Na+ stabilized form We observed that both the ribosyl conformation and the coordination of the carbonyl groups depend on the nature of the the cation. The C-6=O stretching frequency indicates that Na+ coordinates much more strongly to the carbonyl groups than K+ (1672 cm(-1) Na+ vs 1684 cm(-1) K+ at 4 degrees C). Conformationally sensitive modes of the phosphate backbone and the ribosyl ring indicate that Na+ stabilized poly(rI) predominantly exists in a C-3'-endo ribose conformation, whereas K+ stabilized poly(rI) adopts a C-2'-endo conformation possibly as a consequence of the larger ionic radius of the K+ ion. (C) 1998 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 12:00:30