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Titolo:
TUMORS OF THE RETINAL-PIGMENT EPITHELIUM METASTASIZE TO INGUINAL LYMPH-NODES AND SPLEEN IN TYROSINASE-RELATED PROTEIN-1 SV40 T-ANTIGEN TRANSGENIC MICE
Autore:
PENNA D; SCHMIDT A; BEERMANN F;
Indirizzi:
SWISS INST EXPT CANC RES,CHEMIN BOVERESSES 155 CH-1066 EPALINGES SWITZERLAND SWISS INST EXPT CANC RES CH-1066 EPALINGES SWITZERLAND
Titolo Testata:
Oncogene
fascicolo: 20, volume: 17, anno: 1998,
pagine: 2601 - 2607
SICI:
0950-9232(1998)17:20<2601:TOTREM>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
MALIGNANT SKIN MELANOMA; FUSION GENE; MOUSE; PROMOTER; EXPRESSION; ADENOCARCINOMA; MELANOCYTES; HYPERPLASIA; ACTIVATION; SURVIVAL;
Keywords:
METASTASIS; PIGMENTATION; RETINAL PIGMENT EPITHELIUM; TRANSGENIC MICE; TRP-1; TYROSINASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
D. Penna et al., "TUMORS OF THE RETINAL-PIGMENT EPITHELIUM METASTASIZE TO INGUINAL LYMPH-NODES AND SPLEEN IN TYROSINASE-RELATED PROTEIN-1 SV40 T-ANTIGEN TRANSGENIC MICE", Oncogene, 17(20), 1998, pp. 2601-2607

Abstract

The pigment epithelium of the retina (RPE) is derived from the optic cup and is essential for function and development of the eye. We produced a transgenic mouse line that expresses simian virus (SV40) transforming sequences under control of the 1.4 kb tyrosinase-related protein1 (TRP-1) promoter, targeting expression of T antigen (Tag) to the RPE. In transgenic embryos, RPE cells proliferated in the anterior part of the eye and near the optic nerve. This resulted in formation of tumors, which were pigmented and of epithelial origin. In 3 months-old mice, pigmented cells were detected in spleen and inguinal lymph nodes. In spleen, tyrosinase, TRP-1 and SV40 Tag were expressed and tyrosinase was enzymatically active, Pigmented regions were positive for an epithelial marker, cytokeratin. Cell lines were established from tumor and metastases and kept in culture for more than 2 months. These were pigmented, and maintained expression of tyrosinase, TRP-1, cytokeratin and SV40 Tag. This demonstrates that RPE tumor cells metastasize to lymph node and spleen. In conclusion, the metastasis from TRP-1/Tag RPE tumors towards spleen and lymph nodes serves as potential tool to investigate biology and metastasis of tumors derived from the pigment epithelium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 20:07:29