Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
OXIDATIVE BIODEGRADATION OF PHOSPHOROTHIOLATES BY FUNGAL LACCASE
Autore:
AMITAI G; ADANI R; SODMORIAH G; RABINOVITZ I; VINCZE A; LEADER H; CHEFETZ B; LEIBOVITZPERSKY L; FRIESEM D; HADAR Y;
Indirizzi:
ISRAEL INST BIOL RES,DEPT PHARMACOL,POB 19 IL-74100 NESS ZIONA ISRAEL HEBREW UNIV JERUSALEM,FAC AGR IL-76100 REHOVOT ISRAEL
Titolo Testata:
FEBS letters
fascicolo: 3, volume: 438, anno: 1998,
pagine: 195 - 200
SICI:
0014-5793(1998)438:3<195:OBOPBF>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
ORGANOPHOSPHORUS NERVE AGENTS; BACTERIAL ENZYME; DECONTAMINATION; LIGNIN;
Keywords:
ENZYMATIC OXIDATION; LACCASE; 2,2'-AZINOBIS(3-ETHYLBENZTHIAZOLINE-6-SULFONATE); PHOSPHOROTHIOLATE; O-ETHYL [N,N-DIISOPROPYLAMINOETHYL]METHYLPHOSPHONOTHIOLATE; O-ISOBUTYL S-[N,N-DIETHYLAMINOETHYL]METHYLPHOSPHONOTHIOLATE; DIISOPROPYL-AMITON; PLEUROTUS OSTREATUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
G. Amitai et al., "OXIDATIVE BIODEGRADATION OF PHOSPHOROTHIOLATES BY FUNGAL LACCASE", FEBS letters, 438(3), 1998, pp. 195-200

Abstract

Organophosphorus (OP) insecticides and nerve agents that contain P-S bond are relatively more resistant to enzymatic hydrolysis. Purified phenol oxidase (laccase) from the white rot fungus Pleurotus ostreatus (Po) together with the mediator 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) displayed complete and rapid oxidative degradation of the nerve agents VX and Russian VX (RVX) and the insecticide analog diisopropyl-Amiton with specific activity: k(sp) = 2200, 667 and 1833 nmol min(-1) mg(-1), respectively (pH 7.4, 37 degrees C). A molar ratio of 1:20 for OP/ABTS and 0.05 M phosphate at pH 7.4 provided the highest degradation rate of VX and RVX, The thermostable laccase purified from the fungus Chaetomium thermophilium (Ct) in the presence of ARTS caused a 52-fold slower degradation of VX with k(sp) = 42 nmol min(-1)mg(-1). The enzymatic biodegradation products were identified by P-31-NMR and GC/MS analysis. (C) 1998 Federation of European Biochemical Societies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 19:27:45