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Titolo:
DETECTION OF DIGITALIS COMPOUNDS USING A SURFACE-PLASMON RESONANCE-BASED BIOSENSOR
Autore:
LARICCHIAROBBIO L; SILVANA B; GHIONE S; MONTALI U; REVOLTELLA RP;
Indirizzi:
CNR,INST MUTAGENESIS & DIFFERENTIAT,VIA SVEZIA 2-A I-56124 PISA ITALY CNR,INST MUTAGENESIS & DIFFERENTIAT I-56124 PISA ITALY CNR,INST CLIN PHYSIOL I-56100 PISA ITALY UNIV PISA,DEPT SCI MAN & ENVIRONM PISA ITALY
Titolo Testata:
Biosensors & bioelectronics
fascicolo: 10, volume: 13, anno: 1998,
pagine: 1055 - 1060
SICI:
0956-5663(1998)13:10<1055:DODCUA>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOGENOUS OUABAIN; IMMUNOREACTIVE FACTOR; STRUCTURAL-ANALYSIS; PURIFICATION; HYPERTENSION; PRESSURE; NEWBORN; BINDING; BLOOD;
Keywords:
ANTIOUABAIN ANTIBODIES; BIA TECHNOLOGY; ENDOGENOUS DIGITALIS-LIKE FACTOR; RIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
L. Laricchiarobbio et al., "DETECTION OF DIGITALIS COMPOUNDS USING A SURFACE-PLASMON RESONANCE-BASED BIOSENSOR", Biosensors & bioelectronics, 13(10), 1998, pp. 1055-1060

Abstract

An automated surface plasmon resonance-based biosensor system has been used to detect endogenous and exogenous digitalislike factors (EDLF)in the pmolar range in real time. EDLF was purified from umbilical cord blood. EDLF has been suggested to play a role in hypertension and in perinatal adaptation. Highly specific polyclonal anti-ouabain antibodies showed a high affinity binding capacity for ouabain, ouabagenin and strophantidin with an IC50 value of 5 X 10(-10) M, 7.0 X 10(-10) M and 2 X 10(-8) M, respectively. EDLF cross-reacted with antibodies andits concentration in plasma at IC50 was around 50 pmol ouabain equivalent. This study shows the potential usefulness of the biosensor technology for biomolecular interaction analysis. The features of this technology (fully automated, measured in real time, sharpened response) offer several advantages compared with a traditional immunoassay like radioimmunoassay (RIA) in the detection of digitalis compounds in human fluids. (C) 1998 Elsevier Science S.A. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 20:46:43