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Titolo:
ENANTIOSELECTIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION OF METHAMPHETAMINE AND ITS METABOLITES IN HUMAN URINE
Autore:
ALDIRBASHI O; KURODA N; MENICHINI F; NODA S; MINEMOTO M; NAKASHIMA K;
Indirizzi:
NAGASAKI UNIV,SCH PHARMACEUT SCI,1-14 BUNKYO MACHI NAGASAKI 8528521 JAPAN NAGASAKI UNIV,SCH PHARMACEUT SCI NAGASAKI 8528521 JAPAN UNIV CALABRIA,DEPT PHARMACEUT SCI I-87036 RENDE ITALY KYUSHU KOUSEI NENKIN HOSP,DEPT NEUROL KITAKYUSHU FUKUOKA 8068501 JAPAN KYUSHU KOUSEI NENKIN HOSP,DEPT PHARM KITAKYUSHU FUKUOKA 8068501 JAPAN
Titolo Testata:
Analyst (London. 1877. Print)
fascicolo: 11, volume: 123, anno: 1998,
pagine: 2333 - 2337
SICI:
0003-2654(1998)123:11<2333:EHLWF>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
OPTICAL ISOMERS; RAT URINE; AMPHETAMINE; DERIVATIZATION; ENANTIOMERS; SEPARATION; HPLC; PHASE; DRUG;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
O. Aldirbashi et al., "ENANTIOSELECTIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION OF METHAMPHETAMINE AND ITS METABOLITES IN HUMAN URINE", Analyst (London. 1877. Print), 123(11), 1998, pp. 2333-2337

Abstract

A simple, rapid and highly sensitive high-performance liquid chromatographic method with fluorescence detection for determining the enantiomers of methamphetamine and its major metabolites, amphetamine and p-hydroxymethamphetamine, in urine samples was developed. Using a newly developed reagent for amines, namely, 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride, six enantiomers were derivatized under mild conditions (i.e., 10 min at room temperature, pH 9.0) and separated isocratically on a cellulose tris(3,5-dimethylphenylcarbamate) coated silica gelcolumn following a pre-separation on an ODS column within 42 min, andthe effluent was monitored at 440 nm (lambda(ex) 330 nm). Calibrationcurves for these derivatives using spiked human urine were linear in the range 0.05-100 mu mol dm(-3) with correlation coefficients greaterthan or equal to 0.999. The detection limits at a signal-to-noise ratio of 3 were 2.8-8.8 fmol per 5 mu l injection. The relative standard deviations of within- (n = 6) and between-day (n = 5) variations were less than or equal to 7.4%. The method was successfully applied to discriminate between (S)-(+)-methamphetamine and its corresponding metabolites found in abusers' urine and their antipodes in a sample taken from a Parkinsonian patient on selegiline (Deprenyl) therapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 21:36:41