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Titolo:
SELECTIVE INCREASE IN SPECIFIC ALTERNATIVE SPLICE VARIANTS OF TYROSINASE IN MURINE MELANOMAS - A PROJECTED BASIS FOR IMMUNOTHERAPY
Autore:
LEFUR N; KELSALL SR; SILVERS WK; MINTZ B;
Indirizzi:
FOX CHASE CANC CTR,INST CANC RES,7701 BURHOLME AVE PHILADELPHIA PA 19111 FOX CHASE CANC CTR,INST CANC RES PHILADELPHIA PA 19111
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 10, volume: 94, anno: 1997,
pagine: 5332 - 5337
SICI:
0027-8424(1997)94:10<5332:SIISAS>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-INFILTRATING LYMPHOCYTES; MALIGNANT SKIN MELANOMA; CYTOLYTIC T-LYMPHOCYTES; HLA-A2 MELANOMAS; TRANSGENIC MICE; CELL-LINES; GENE; ANTIGEN; IDENTIFICATION; EXPRESSION;
Keywords:
TRANSGENIC MOUSE MODELS; RNA PROCESSING; MELANOCYTIC GENES; MELANOMA PROGRESSION; MELANOMA METASTASIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
N. Lefur et al., "SELECTIVE INCREASE IN SPECIFIC ALTERNATIVE SPLICE VARIANTS OF TYROSINASE IN MURINE MELANOMAS - A PROJECTED BASIS FOR IMMUNOTHERAPY", Proceedings of the National Academy of Sciences of the United Statesof America, 94(10), 1997, pp. 5332-5337

Abstract

Melanomas tend to become less pigmented in the course of malignant progression, Thus, as proliferation increases, the tumors are decreasingly characterized by the tissue-specific phenotype of normally differentiated melanocytes, To learn whether the decline in melanization is associated with a shift from constitutive to alternative splicing of some pigment gene pre-mRNAs, melanomas were collected from Tyr-SV40E transgenic mice of the standard C57BL/6 strain, The mRNAs of the tyrosinase gene, which has a key role in melanogenesis, were analyzed by quantitative reverse transcriptase-PCR in 34 samples from 16 cutaneous tumors and 9 metastases, The cutaneous tumors included some cases with distinct melanotic and amelanotic zones, which were separately analyzed, All tyrosinase transcripts found in the melanomas were also found in normal skin melanocytes. However, the Delta 1b and Delta 1d alternatively spliced transcripts, due to deletions within the first exon, were specifically augmented in most of the tumors over their very low levels in skin; the exceptions were some all-amelanotic tumors in which no tyrosinase transcripts were detected, The level of Delta 1b rose as highas 11.3% of total tyrosinase mRNAs as compared with 0.6% in skin; Delta 1d reached 4.0% as compared with 0.8% in skin, Expression of these splice variants was highest in the melanotic components of zonal primary tumors, relatively lower in their amelanotic components, and still lower in all-amelanotic primary tumors and amelanotic metastases, The increase in Delta 1b and Delta 1d transcripts may be predicted to increase the levels of unusual peptides, which could have antigenic potential in the tumors, especially in the relatively early phases of malignancy, Analyses of the alternative transcripts of other pigment genes may identify additional candidate antigens, ultimately enabling melanoma cells in all phases of the disease to be represented as a basis for immune intervention.

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Documento generato il 04/12/20 alle ore 19:37:55