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Titolo:
LIGAND-DEPENDENT DEVELOPMENT OF THE ENDOTHELIAL AND HEMATOPOIETIC LINEAGES FROM EMBRYONIC MESODERMAL CELLS EXPRESSING VASCULAR ENDOTHELIAL GROWTH-FACTOR RECEPTOR-2
Autore:
EICHMANN A; CORBEL C; NATAF V; VAIGOT P; BREANT C; LEDOUARIN NM;
Indirizzi:
CNRS,INST EMBRYOL CELLULAIRE & MOL,49BIS AVE BELLE GABRIELLE F-94736 NOGENT SUR MARNE FRANCE CNRS,INST EMBRYOL CELLULAIRE & MOL F-94736 NOGENT SUR MARNE FRANCE COLL FRANCE F-94736 NOGENT SUR MARNE FRANCE
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 10, volume: 94, anno: 1997,
pagine: 5141 - 5146
SICI:
0027-8424(1997)94:10<5141:LDOTEA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
TYROSINE KINASE; VASCULOGENESIS; HEMATOPOIESIS; THROMBOCYTES; FLT-1; FLK-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
A. Eichmann et al., "LIGAND-DEPENDENT DEVELOPMENT OF THE ENDOTHELIAL AND HEMATOPOIETIC LINEAGES FROM EMBRYONIC MESODERMAL CELLS EXPRESSING VASCULAR ENDOTHELIAL GROWTH-FACTOR RECEPTOR-2", Proceedings of the National Academy of Sciences of the United Statesof America, 94(10), 1997, pp. 5141-5146

Abstract

The existence of a common precursor for endothelial and hemopoietic cells, termed the hemangioblast, has been postulated since the beginning of the century, Recently, deletion of the endothelial-specific vascular endo thelial growth factor receptor 2 (VEGFR2) by gene targeting has shown that both endothelial and hemopoietic cells are absent in homozygous null mite, This observation suggested that VEGFR2 could be expressed by the hemangioblast and essential for its further differentiation along both lineages, However, it was not possible to exclude the hypothesis that hemopoietic failure was a secondary effect resulting from the absence of an endothelial cell microenvironment. To distinguishbetween these two hypotheses, we have produced a mAb directed againstthe extracellular domain of avian VEGFR2 and isolated VEGFR2+ cells from the mesoderm of chicken embryos at the gastrulation stage, We havefound that in clonal cultures, a VEGPR2+ cell gives rise to either a hemopoietic or an endothelial cell colony. The developmental decision appears to be regulated by the binding of two different VEGFR2 ligands, Thus, endothelial differentiation requires VEGF, whereas hemopoieticdifferentiation occurs in the absence of VEGF and is significantly reduced by soluble VEGFR2, showing that this process could be mediated by a second, yet unidentified, VEGFR2 ligand, These observations thus suggest strongly that in the absence of the VEGFR2 gene product, the precursors of both hemopoietic and vascular endothelial lineages cannot survive. These cells therefore might be the initial targets of the VEGFR2 null mutation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 09:49:37