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Titolo:
IN-UTERO TRANSPLANTATION OF HUMAN FETAL HEMATOPOIETIC-CELLS IN NOD SCID MICE/
Autore:
TURNER CW; ARCHER DR; WONG J; YEAGER AM; FLEMING WH;
Indirizzi:
OREGON HLTH SCI UNIV,DIV HEMATOL & MED ONCOL,RM 5330,BASIC SCI BLDG L580,3181 SW SAM JACKSON P PORTLAND OR 97201 OREGON HLTH SCI UNIV,DIV HEMATOL & MED ONCOL PORTLAND OR 97201 EMORY UNIV,SCH MED,DEPT PEDIAT ATLANTA GA 00000 EMORY UNIV,SCH MED,WINSHIP CANC CTR ATLANTA GA 00000
Titolo Testata:
British Journal of Haematology
fascicolo: 2, volume: 103, anno: 1998,
pagine: 326 - 334
SICI:
0007-1048(1998)103:2<326:ITOHFH>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC STEM-CELLS; SEVERE COMBINED IMMUNODEFICIENCY; ADULT BONE-MARROW; IN-UTERO; INUTERO TRANSPLANTATION; PROGENITOR CELLS; T-CELL; ENGRAFTMENT; CHIMERISM; SHEEP;
Keywords:
IN UTERO TRANSPLANTATION; HUMAN; NOD/SCID MOUSE; XENOGRAFT; HEMATOPOIESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
C.W. Turner et al., "IN-UTERO TRANSPLANTATION OF HUMAN FETAL HEMATOPOIETIC-CELLS IN NOD SCID MICE/", British Journal of Haematology, 103(2), 1998, pp. 326-334

Abstract

We have previously demonstrated that high levels of allogeneic, donor-derived mouse haemopoietic progenitor cells engraft following in utero transplantation in NOD/SCID mice. To evaluate whether the fetal NOD/SCID haemopoietic microenvironment supports the growth and developmentof human fetal haemopoietic progenitor cells, we injected fetal livermononuclear cells (FL) or fetal bone marrow (FBM) derived CD34(+) cells into NOD/SCID mice on day 13/14 of gestation. At 8 weeks of age 12%of FBM recipients and 10% of FL recipients were found to have been successfully engrafted with CD45(+) human cells. CD45(+) cells were present in the BM of all chimaeric animals; 5/6 recipients showed engraftment of the spleen, and 4/6 recipients had circulating human cells in the peripheral blood (PB). The highest levels of donor cells were foundin the BM, with up to 15% of the nucleated cells expressing human specific antigens. Multilineage human haemopoietic engraftment, includingB cells (CD19), myelomonocytic cells (CD13/33) and haemopoietic progenitor cells (CD34), was detected in the BM of chimaeric mice. In contrast, no human CD3(+) cells were detected in any of the tissues evaluated. When the absolute number of engrafted human cells in the PB, BM and spleens of chimaeric mice was determined, a mean 16-fold expansion of human donor cells was observed. Although multilineage engraftment occurs in these fetal recipients, both the frequency and the levels of engraftment are lower than those previously reported when human cells are transplanted into adult NOD/SCID recipients.

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Documento generato il 26/09/20 alle ore 18:25:29