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Titolo:
MICROENCAPSULATION OF HEPATITIS-B CORE ANTIGEN FOR VACCINE PREPARATION
Autore:
UCHIDA T; SHIOSAKI K; NAKADA Y; FUKADA K; EDA Y; TOKIYOSHI S; NAGAREYA N; MATSUYAMA K;
Indirizzi:
MUKOGAWA WOMENS UNIV,SCH PHARMACEUT SCI,11-68 KOSHIEN 9 BANCHO NISHINOMIYA HYOGO 6638179 JAPAN CHEMOSEROTHERAPEUT RES INST KYOKUSHI KIKUCHI KUMAMOTO 86515 JAPAN TORAY INDUSTRIES LTD KAMAKURA KANAGAWA 248 JAPAN
Titolo Testata:
Pharmaceutical research
fascicolo: 11, volume: 15, anno: 1998,
pagine: 1708 - 1713
SICI:
0724-8741(1998)15:11<1708:MOHCAF>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLY(LACTIDE-CO-GLYCOLIDE) MICROSPHERES; ORAL DELIVERY; OVALBUMIN; RELEASE;
Keywords:
HEPATITIS B CORE ANTIGEN; MICROSPHERES; POLY(LACTIDE-CO-GLYCOLIDE); ELISA; VACCINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
T. Uchida et al., "MICROENCAPSULATION OF HEPATITIS-B CORE ANTIGEN FOR VACCINE PREPARATION", Pharmaceutical research, 15(11), 1998, pp. 1708-1713

Abstract

Purpose. To prepare poly(lactide-co-glycolide)(PLGA) microspheres containing recombinant hepatitis B core antigen (HBcAg; Mw = 3,600,000) by a w/o/w emulsion/solvent evaporation method and evaluate the possibility of this system as a potent long-acting carrier for hepatitis B core antigen in mice. Methods. Various additives had been incorporated in the internal aqueous phase during the process of microencapsulating HBcAg, HBcAg antigenicity in the medium extracted from the prepared microspheres were measured by ELISA. Shape confirmation of the HBcAg antigen was performed by a sucrose gradient velocity centrifugal technique. For in vivo study, prepared microspheres were administered subcutaneously to Balb/C mice, and the serum IgG level was determined by ELISA. Results. The inactivation of HBcAg by methylene chloride was dramatically reduced by the addition of gelatin (4-8% (w/v)) to the internal aqueous phase during the preparation. Further improvement of the loading efficiency to almost 61% resulted with cooling (4 degrees C). The prepared microspheres (4.27 mu m +/- 1.23 mu m) containing 0.15% HBcAg displayed burst release (50-60% within 2 days). In subcutaneous inoculation, the adjuvant effect of PLGA microspheres was almost the same asthat of the complete Freund's adjuvant. Whereas oral inoculation using the microspheres was not effective. Conclusions. The pH of the addedgelatin seemed to be the key to the stabilization of HBcAg from various stability tests and CD spectrum study. Finally, the possibility of using this system as a potent long-acting hepatitis B vaccine was demonostrated.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 03:42:40