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Titolo:
BOTH TNF RECEPTORS ARE REQUIRED FOR DIRECT TNF-MEDIATED CYTOTOXICITY IN MICROVASCULAR ENDOTHELIAL-CELLS
Autore:
LUCAS R; GARCIA I; DONATI YRA; HRIBAR M; MANDRIOTA SJ; GIROUD C; BUURMAN WA; FRANSEN L; SUTER PM; NUNEZ G; PEPPER MS; GRAU GE;
Indirizzi:
UNIV GENEVA,MED CTR,DEPT INTERNAL MED,LAB INTENS CARE RES,RUE MICHEL SERVET 1 CH-1211 GENEVA 4 SWITZERLAND UNIV GENEVA,MED CTR,DEPT ANAESTHESIOL PHARMACOL & SURG INTENS CARE,IMMUNOPATHOL LAB CH-1211 GENEVA SWITZERLAND UNIV GENEVA,MED CTR,DEPT PATHOL CH-1211 GENEVA SWITZERLAND UNIV GENEVA,MED CTR,DEPT MORPHOL CH-1211 GENEVA SWITZERLAND UNIV LIMBURG,DEPT SURG NL-6200 MD MAASTRICHT NETHERLANDS INNOGENET GHENT BELGIUM UNIV MICHIGAN,SCH MED ANN ARBOR MI 48109 UNIV MEDITERRANEE,FAC MED,CNRS UPRES A6020 MARSEILLE FRANCE
Titolo Testata:
European Journal of Immunology
fascicolo: 11, volume: 28, anno: 1998,
pagine: 3577 - 3586
SICI:
0014-2980(1998)28:11<3577:BTRARF>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; EXPERIMENTAL CEREBRAL MALARIA; FACTOR-ALPHA; APOPTOSIS; DEATH; ACTIVATION; MICE; EXPRESSION; INFECTION; TOXICITY;
Keywords:
TNF; ENDOTHELIAL CELL; APOPTOSIS; BCL-XL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
R. Lucas et al., "BOTH TNF RECEPTORS ARE REQUIRED FOR DIRECT TNF-MEDIATED CYTOTOXICITY IN MICROVASCULAR ENDOTHELIAL-CELLS", European Journal of Immunology, 28(11), 1998, pp. 3577-3586

Abstract

The conditions under which tumor necrosis factor-alpha (TNF) induces apoptosis in primary microvascular endothelial cells (MVEC) were investigated. In the absence of sensitizing agents, TNF induced apoptosis after 3 days of incubation in confluent MVEC. In contrast, upon addition of the transcriptional inhibitor actinomycin D (Act. D), confluence was no longer required and apoptosis occurred already after 16 h. To assess the role of either TNF receptor (TNFR) type in apoptosis, MVEC isolated from mice genetically deficient in TNFR1 (Tnfr1 degrees mice) or TNFR2 (Tnfr2 degrees mice) were incubated with TNF in the presence or absence of Act. D. Under sensitized conditions, Tnfr2 degrees MVEC were lysed like controls, whereas Tnfr1 degrees MVEC were completely resistant, indicating an exclusive role for TNFR1. In contrast, in the absence of Act. D, confluent monolayers of wild-type cells were lysed by TNF, but both Tnfr1 degrees and Tnfr2 degrees MVEC were resistant to TNF-mediated toxicity, indicating a requirement for both TNFR types. Overexpression of the anti-apoptotic protein bcl-xL in MVEC led to a protection against the direct, but not the sensitized cytotoxicity of TNF. In conclusion, in pathophysiologically relevant conditions, both TNFR appear to be required for TNF-induced apoptosis in MVEC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 21:02:35