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Titolo:
FETAL CELLS IN MATERNAL BLOOD - THE USE OF PRIMED IN-SITU (PRINS) LABELING TECHNIQUE FOR FETAL CELL DETECTION AND SEX ASSESSMENT
Autore:
ORSETTI B; LEFORT G; BOULOT P; ANDREO B; PELLESTOR F;
Indirizzi:
CNRS,CRBM,UPR 9008,BP 5051,ROUTE MENDE F-34033 MONTPELLIER FRANCE CNRS,CRBM,UPR 9008 F-34033 MONTPELLIER FRANCE ARNAUD VILLENEUVE HOSP,CYTOGENET LAB F-34295 MONTPELLIER 5 FRANCE ARNAUD VILLENEUVE HOSP,DEPT GYNAECOL OBSTET F-34295 MONTPELLIER 5 FRANCE
Titolo Testata:
Prenatal diagnosis
fascicolo: 10, volume: 18, anno: 1998,
pagine: 1014 - 1022
SICI:
0197-3851(1998)18:10<1014:FCIMB->2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALPHA-SATELLITE DNA; PRENATAL-DIAGNOSIS; PERIPHERAL-BLOOD; HUMAN-CHROMOSOMES; IN-SITU; NUCLEATED ERYTHROCYTES; INSITU; IDENTIFICATION; GRANULOCYTES; SEQUENCES;
Keywords:
FETAL CELLS; MATERNAL BLOOD; PRENATAL DIAGNOSIS; NONINVASIVE; PRIMED IN SITU LABELING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
B. Orsetti et al., "FETAL CELLS IN MATERNAL BLOOD - THE USE OF PRIMED IN-SITU (PRINS) LABELING TECHNIQUE FOR FETAL CELL DETECTION AND SEX ASSESSMENT", Prenatal diagnosis, 18(10), 1998, pp. 1014-1022

Abstract

Prenatal diagnosis is presently performed following invasive procedures with variable risks of fetal loss; non-invasive procedures using fetal cells in maternal blood would be welcome for the early detection of fetal sex or aneuploidy. We describe a simple and rapid protocol to detect fetal cells and thus to assess fetal sex. In a first step, nucleated blood cells were separated into mononuclear and polynuclear cells using a double density gradient centrifugation. In a second step primed in situ (PRINS) labelling technique was performed to label Y-chromosomes. 15 samples were studied and correct gender assignment was madein 13/15. The number of labelled nuclei was higher in polynuclear cell phases than in mononuclear cell phases. Moreover, the polylobular aspect of labelled nuclei from polynuclear cell phases strongly suggested that they could belong to fetal polynuclear cells. The PRINS technique combines some advantages of FISH, such as visual assessment of iii situ chromosome labelling and the powerful specificity and sensitivityof PCR. In association with a simple enrichment procedure it constitutes a rapid protocol for fetal cell detection, non-invasive early prenatal sex assessment, and could further be applied to detect the main viable aneuploidies. (C) 1998 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 16:40:55