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Titolo:
SCLERODERMA FIBROBLASTS PROMOTE MIGRATION OF MONONUCLEAR LEUKOCYTES ACROSS ENDOTHELIAL-CELL MONOLAYERS
Autore:
DENTON CP; SHIWEN X; SUTTON A; ABRAHAM DJ; BLACK CM; PEARSON JD;
Indirizzi:
ROYAL FREE HOSP,ACAD UNIT RHEUMATOL,POND ST LONDON NW3 2QG ENGLAND ROYAL FREE HOSP,ACAD UNIT RHEUMATOL LONDON NW3 2QG ENGLAND UNIV LONDON KINGS COLL,VASC BIOL RES CTR LONDON WC2R 2LS ENGLAND
Titolo Testata:
Clinical and experimental immunology
fascicolo: 2, volume: 114, anno: 1998,
pagine: 293 - 300
SICI:
0009-9104(1998)114:2<293:SFPMOM>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYSTEMIC-SCLEROSIS; IN-VITRO; NEUTROPHIL MIGRATION; ALPHA-CHEMOKINE; ADHESION; SKIN; INTERLEUKIN-8; EXPRESSION; INDUCTION; LINES;
Keywords:
SCLERODERMA; SYSTEMIC SCLEROSIS; ENDOTHELIUM; FIBROBLAST; COCULTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
C.P. Denton et al., "SCLERODERMA FIBROBLASTS PROMOTE MIGRATION OF MONONUCLEAR LEUKOCYTES ACROSS ENDOTHELIAL-CELL MONOLAYERS", Clinical and experimental immunology, 114(2), 1998, pp. 293-300

Abstract

Perivascular infiltrates of inflammatory cells are a hallmark of lesional skin in scleroderma. We have explored the potential for scleroderma fibroblasts to modulate mononuclear leucocyte migration across endothelial cell monolayers in tissue culture, and to regulate expression of endothelial cell adhesion molecules. Fibroblasts were grown from skin biopsies of eight patients with active diffuse cutaneous scleroderma and from four healthy controls. Go-culture and conditioned medium transfer experiments examined the effect of soluble fibroblast products on mononuclear leucocyte (U937) cell migration across endothelial cell(IE-7) monolayers grown on tissue culture inserts. Go-culture of scleroderma, but not control fibroblasts, promoted transendothelial migration of U937 cells. Scleroderma fibroblast-conditioned medium had qualitatively similar effects and equivalent results were obtained using Jurkat-6 (T lymphocyte) cells, and with peripheral blood mononuclear cells from a patient with diffuse cutaneous scleroderma. Promotion of leucocyte migration does not appear to result from increased endothelial adhesion molecule expression, since fibroblast-conditioned medium did not up-regulate endothelial cell expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) or E-selectin. Moreover, leucocyte migration across cytokine-activated endothelial cell layers in co-cuture with fibroblasts was less than across resting cells, although the selective effect of scleroderma fibroblastco-culture persisted. Recombinant monocyte chemoattractant protein-1 (MCP-1) or IL-8 increased passage of mononuclear leucocytes across endothelial cell monolayers, whilst anti-MCP-l, but not anti-IL-8 antibodies, significantly reduced the effect of fibroblast conditioned medium. These data suggest that systemic sclerosis (SSc) fibroblasts promoteleucocyte migration across endothelial cell monolayers in tissue culture via an MGP-1-dependent mechanism. These findings may be relevant to the perivascular mononuclear leucocyte infiltrates characteristic ofearly SSc lesions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 01:10:16