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Titolo:
AN A G-RICH MOTIF IN THE RAT FIBROBLAST GROWTH FACTOR-2 GENE CONFERS ENHANCER ACTIVITY ON A HETEROLOGOUS PROMOTER IN NEONATAL RAT CARDIAC MYOCYTES/
Autore:
DETILLIEUX KA; MEYERS AFA; MEIJ JTA; CATTINI PA;
Indirizzi:
UNIV MANITOBA,GENE TECHNOL GRP,730 WILLIAM AVE WINNIPEG MB R3E 3J7 CANADA UNIV MANITOBA,GENE TECHNOL GRP WINNIPEG MB R3E 3J7 CANADA UNIV MANITOBA,DEPT PHYSIOL WINNIPEG MB R3E 3J7 CANADA UNIV MANITOBA,DEPT PHARMACOL & THERAPEUT WINNIPEG MB R3E 3J7 CANADA
Titolo Testata:
Molecular and cellular biochemistry
fascicolo: 1-2, volume: 188, anno: 1998,
pagine: 169 - 176
SICI:
0300-8177(1998)188:1-2<169:AAGMIT>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR-II; FACTOR TRANSCRIPTION; CELLS; PROLIFERATION; HEART; RECEPTOR; INHIBITION; RELEASE; ROLES;
Keywords:
FGF-2; TRANSCRIPTION; GENE TRANSFER; HSV-THYMIDINE KINASE PROMOTER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
K.A. Detillieux et al., "AN A G-RICH MOTIF IN THE RAT FIBROBLAST GROWTH FACTOR-2 GENE CONFERS ENHANCER ACTIVITY ON A HETEROLOGOUS PROMOTER IN NEONATAL RAT CARDIAC MYOCYTES/", Molecular and cellular biochemistry, 188(1-2), 1998, pp. 169-176

Abstract

We have cloned the rat fibroblast growth factor-2 (FGF-2) promoter region including 1058 base pairs (bp) of 5'-flanking DNA. Complete sequencing of this promoter region revealed a 74 bp domain between nucleotides -793 and -720 that was greater than 97% A/G-rich. A repeat of the sequence 5'-AGGGAGGG-3' separated by 11 bp was located at the core of this domain. A 37 bp A/G-rich oligonucleotide containing these AGGG-repeat sequences was synthesised, and tested for function on a minimal herpes simplex virus thymidine kinase (TK) promoter, fused to the firefly luciferase gene (TKp.luc), in transiently transfected neonatal rat cardiac myocytes. Promoter activity was stimulated similar to 3 fold in the presence of AGGG-repeat sequences. This effect was neither tissue or species-specific since TK promoter activity was increased similarto 11 fold in both rat and human glial tumor cells. Four specific complexes (C1-4) were detected between neonatal rat heart nuclear proteins and the 37 bp A/G-rich oligonucleotide by gel mobility shift assay. Competition with excess unlabelled 37 bp A/G-rich oligonucleotide revealed that two complexes represented very high affinity/specificity interactions (C2 > C4) while C1 and C3 were of lower affinity.. As a result, competition with up to a 25 fold molar excess of 37 bp A/G-rich oligonucleotide led to the loss of C2 and C4, and a corresponding and transient increase in the levels of C1 and C3, which themselves were reduced with more competitor oligonucleotide. The AGGG-repeat resembles the 5'-gGGGAGGG-3' sequence previously implicated in the response of the atrial natriuretic factor promoter to the a-adrenergic agonist, phenylephrine. Although an additional 1.5 fold increase in TK promoter activity was detected in the presence of the 37 bp A/G-rich oligonucleotide with phenylephrine treatment of transfected myocytes, this effect was not statistically significant. Furthermore, there was no differencein the gel mobility shift (C1-4) pattern obtained with the 37 bp A/G-rich oligonucleotide and nuclear protein isolated from neonatal rat cardiac myocytes grown in the presence or absence of norepinephrine. These data suggest that the A/G rich sequences in the rat FGF-2 gene 5'-flanking DNA, including the AGGG-repeat, are able to confer stimulatoryactivity on a promoter in a tissue- and species-independent manner, but alone are not able to induce a significant phenylephrine response in neonatal rat cardiac myocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 04:20:46