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Titolo:
THE DUAL PHASES OF THE RESPONSE TO NEONATAL EXPOSURE TO A V-H FAMILY-RESTRICTED STAPHYLOCOCCAL B-CELL SUPERANTIGEN
Autore:
SILVERMAN GJ; NAYAK JV; WARNATZ K; HAJJAR FF; CARY S; TIGHE H; CURTISS VE;
Indirizzi:
UNIV CALIF SAN DIEGO,DEPT MED 0663,SAM & ROSE STEIN INST RES AGING,9500 GILMAN DR LA JOLLA CA 92093 UNIV CALIF SAN DIEGO,DEPT MED,THEODORE GILDRED CANC CTR LA JOLLA CA 92093
Titolo Testata:
The Journal of immunology (1950)
fascicolo: 10, volume: 161, anno: 1998,
pagine: 5720 - 5732
SICI:
0022-1767(1998)161:10<5720:TDPOTR>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
IG-SECRETING CELLS; PROTEIN-A; T-CELLS; IN-VIVO; TOLERANCE INDUCTION; SELF-TOLERANCE; SURFACE-IMMUNOGLOBULIN; DENDRITIC CELLS; SOLUBLE-ANTIGEN; MICE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
97
Recensione:
Indirizzi per estratti:
Citazione:
G.J. Silverman et al., "THE DUAL PHASES OF THE RESPONSE TO NEONATAL EXPOSURE TO A V-H FAMILY-RESTRICTED STAPHYLOCOCCAL B-CELL SUPERANTIGEN", The Journal of immunology (1950), 161(10), 1998, pp. 5720-5732

Abstract

In vitro studies of several naturally occurring proteins have characterized V-H family-specific B lymphocyte binding and stimulatory properties that appear analogous to those of T cell superantigens. To examine the in vivo consequences of exposure to a putative B cell superantigen, we treated neonatal BALB/c mice with a form of staphylococcal protein A (MS) devoid of Fc gamma binding activity, which retains the dan VHIII Fab binding specificity. In naive adults, about 5% of peripheralB cells and > 13% of splenic IgM-secreting cells display MS binding activity, in association with high IgM and low IgG circulating anti-MS Ab titers. Neonatal exposure to MS elicited two distinct temporal phases of immune responsiveness. The early phase, representing the first approximately 5 wk of life, was associated with MS-specific B cell and T cell tolerance. Microfluorometric assays revealed that exposure caused a dramatic MS-specific B cell clonal loss in bone marrow and spleen, but levels normalized by about 3 wk of life. The late phase (>6 wk of age) was associated with spontaneous priming for MS-specific T cell responses and production of MS-specific IgG1 Abs despite long term persistently depressed in vivo and in vitro MS-specific IgM responses. Invivo challenge during the late phase induced high frequencies of MS-specific IgG-secreting cells, indicating recruitment of highly focused Ab responses that were predominantly encoded by rearrangements of the S107 family, a member of the VHIII clan. These studies document the immunodominance of the V-H-restricted Fab binding site on staphylococcalprotein A and demonstrate the diverse effects of a B cell superantigen on the emerging peripheral B cell compartment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 20:19:06