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Titolo:
SHORT ALLELE DOMINANCE AS A SOURCE OF HETEROZYGOTE DEFICIENCY AT MICROSATELLITE LOCI - EXPERIMENTAL-EVIDENCE AT THE DINUCLEOTIDE LOCUS GV1CT IN GRACILARIA-GRACILIS (RHODOPHYTA)
Autore:
WATTIER R; ENGEL CR; SAUMITOULAPRADE P; VALERO M;
Indirizzi:
UNIV LILLE 1,LAB GENET & EVOLUT POPULAT VEGETALES,URA CNRS 1185,GDR CNRS IFREMER 1002,BAT SN2 F-59655 VILLENEUVE DASCQ FRANCE UNIV LILLE 1,LAB GENET & EVOLUT POPULAT VEGETALES,URA CNRS 1185,GDR CNRS IFREMER 1002 F-59655 VILLENEUVE DASCQ FRANCE
Titolo Testata:
Molecular ecology
fascicolo: 11, volume: 7, anno: 1998,
pagine: 1569 - 1573
SICI:
0962-1083(1998)7:11<1569:SADAAS>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
NULL ALLELE; DNA; SEQUENCE; AMPLIFICATION; POPULATIONS; POLYMORPHISM; NUCLEAR; AFRICA; PCR;
Keywords:
ALLELE SIZE; HETEROZYGOTE DEFICIENCY; HAPLO-DIPLOID; NULL ALLELES; MICROSATELLITE LOCI; SHORT ALLELE DOMINANCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
R. Wattier et al., "SHORT ALLELE DOMINANCE AS A SOURCE OF HETEROZYGOTE DEFICIENCY AT MICROSATELLITE LOCI - EXPERIMENTAL-EVIDENCE AT THE DINUCLEOTIDE LOCUS GV1CT IN GRACILARIA-GRACILIS (RHODOPHYTA)", Molecular ecology, 7(11), 1998, pp. 1569-1573

Abstract

In this study, we compared the genotypes obtained at a microsatellitelocus using two methods of amplification and detection of variation in a set of individuals belonging to the red alga haplo-diploid species, Gracilaria gracilis. The methods varied in their capacity to detect longer alleles in heterozygotes, resulting in an apparent heterozygotedeficiency. We attributed this bias in favour of short alleles to competition leading to the preferential amplification of shorter alleles (short allele dominance). To test this hypothesis, we created artificial heterozygotes (mixtures of two haploid DNA samples) and showed thatlong alleles already less intense than short alleles, 'suffer' more from being in association.

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Documento generato il 15/07/20 alle ore 05:15:04