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Titolo:
PROTRUSION, CONTRACTION AND SEGREGATION OF MEMBRANE-COMPONENTS ASSOCIATED WITH PASSIVE DEFORMATION AND SHAPE RECOVERY OF WALKER CARCINOSARCOMA CELLS
Autore:
SCHUTZ K; KELLER H;
Indirizzi:
UNIV BERN,DEPT PATHOL,MURTENSTR 31 CH-3010 BERN SWITZERLAND UNIV BERN,DEPT PATHOL CH-3010 BERN SWITZERLAND
Titolo Testata:
European journal of cell biology
fascicolo: 2, volume: 77, anno: 1998,
pagine: 100 - 110
SICI:
0171-9335(1998)77:2<100:PCASOM>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMORPHONUCLEAR LEUKOCYTES; EPITHELIAL-CELLS; CLEAVAGE FURROW; CYTOCHALASIN-B; ANIMAL-CELLS; LEADING-EDGE; LOCOMOTION; FLOW; NEUTROPHILS; COLCHICINE;
Keywords:
PASSIVE DEFORMATION; CELL SHAPE; PROTRUSIONS; CON A BINDING; CONSTRICTION RING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
K. Schutz e H. Keller, "PROTRUSION, CONTRACTION AND SEGREGATION OF MEMBRANE-COMPONENTS ASSOCIATED WITH PASSIVE DEFORMATION AND SHAPE RECOVERY OF WALKER CARCINOSARCOMA CELLS", European journal of cell biology, 77(2), 1998, pp. 100-110

Abstract

We mimicked essential elements of the cortical-contraction model of cell locomotion by exposing Walker carcinosarcoma cells to passive deformation and to hydrostatic pressure changes within micropipettes followed by shape recovery after release. Protrusion, contraction and segregation of cell surface membrane components were observed. Regardless of the initial shape (spherical, polarized with lamellipodia or blebs) cells tended to produce blebs during uptake into the pipette and during and after release from the pipette, but usually not during the shorttime they were held within the pipette. Bleb formation depended on the deformation stress, extracellular hydrostatic pressure and cell structure (initial shape). In polarized cells, blebs were much more readily induced at the front as compared to the tail. Cells undergoing largedeformations formed constriction rings and large hyaline caps. Deformation resulted in segregation of membrane components. Equatorial constriction rings divided the cell into two parts which differed with respect to Con A binding and their role in shape recovery. Spherical and polarized cells usually recover the respective initial shape. Polarizedcells reacquired the same type of protrusions (blebs or lamellipodia)which they exhibited before deformation. Shape recovery of spherical cells was characterized by a rapid recoil by about 20 % followed by a slow asymptotic recovery.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 08:34:12