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Titolo:
EFFECT OF A CHRONIC STRESS ON CRF NEURONAL-ACTIVITY AND EXPRESSION OFITS TYPE-1 RECEPTOR IN THE RAT-BRAIN
Autore:
BONAZ B; RIVEST S;
Indirizzi:
CHU LAVAL,RES CTR,MOL ENDOCRINOL LAB,2705 BLVD LAURIER QUEBEC CITY PQG1V 4G2 CANADA CHU LAVAL,RES CTR,MOL ENDOCRINOL LAB QUEBEC CITY PQ G1V 4G2 CANADA UNIV LAVAL QUEBEC CITY PQ G1V 4G2 CANADA CHU GRENOBLE,HOP A MICHALLON,INSERM,PHYSIOL LAB,SECT NEUROPHYSIOL F-38043 GRENOBLE 09 FRANCE
Titolo Testata:
American journal of physiology. Regulatory, integrative and comparative physiology
fascicolo: 5, volume: 44, anno: 1998,
pagine: 1438 - 1449
SICI:
0363-6119(1998)44:5<1438:EOACSO>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CORTICOTROPIN-RELEASING-FACTOR; MESSENGER-RIBONUCLEIC-ACID; IMMOBILIZATION STRESS; C-FOS; PARAVENTRICULAR NUCLEUS; DIFFERENTIAL REGULATION; ANTERIOR-PITUITARY; RESTRAINT STRESS; IMMUNE CHALLENGE; GENE-EXPRESSION;
Keywords:
C-FOS; HYPOTHALAMUS; IN SITU HYBRIDIZATION HISTOCHEMISTRY; IMMUNOCYTOCHEMISTRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
B. Bonaz e S. Rivest, "EFFECT OF A CHRONIC STRESS ON CRF NEURONAL-ACTIVITY AND EXPRESSION OFITS TYPE-1 RECEPTOR IN THE RAT-BRAIN", American journal of physiology. Regulatory, integrative and comparative physiology, 44(5), 1998, pp. 1438-1449

Abstract

The purpose of this study was to compare the effect of an acute versus a chronic immobilization stress on the genetic expression of c-fos and corticotropin-releasing factor type 1 receptor (CRF1 receptor) in the paraventricular nucleus (PVN) of the rat hypothalamus. Male Sprague-Dawley rats were exposed to either a single 90-min immobilization stress or the same session for 11 consecutive days. Animals were deeply anesthetized before (control); immediately, 1.5, 3, 6, or 12 h after the acute stress; or after the last session of the repeated exposures toimmobilization. Coronal frozen sections (30 mu m) of the brains were cut and mRNAs encoding the rat c-ibs and CRF1 receptor were assayed byin situ hybridization histochemistry using S-35-labeled riboprobes. Localization of these transcripts within PVN CRF-immunoreactive (ir) neurons was also determined. The expression of the mRNA encoding either c-fos or CRF1 receptor was barely detectable to low in the PVN of control animals, but the acute stress session induced a robust signal for both transcripts in this endocrine nucleus. Numerous CRF-ir neurons were positive for the gene encoding either c-fos or CRF1 receptor in thePVN of acutely stressed animals. In contrast, the PVN of chronically stressed animals displayed a significantly lower CRF1 receptor mRNA signal after the last stress session. In these animals, stress-induced transcription of c-fos mRNA occurred in the magnocellular PVN 90 min after the end of the last stress session but only a low signal was detected in the parvocellular division. Moreover, very few CRF-ir neurons of the PVN expressed either the CRF1 receptor or c-fos transcript in chronically stressed rats. These data provide evidence for an adaptive cellular mechanism involving an attenuated action of CRF within the PVNin response to repeated homotypic stress exposures.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 03:16:49