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Titolo:
ISOLATED PORCINE OVARIAN FOLLICLES AS A MODEL FOR THE STUDY OF HORMONE AND GROWTH-FACTOR ACTION ON OVARIAN SECRETORY ACTIVITY
Autore:
SIROTKIN AV; MAKAREVICH AV; KOTWICA J; MARNET PG; KWON HB; HETENYI L;
Indirizzi:
RES INST ANIM PROD,HLOHOVSKA 2 NITRA 94992 SLOVAKIA INST ANIM REPROD & FOOD RES PL-10718 OLSZTYN POLAND INRA,ENSAR,LAB RECH TRAITE F-35042 RENNES FRANCE CHONNAM NATL UNIV,DEPT BIOL KWANGJU 500757 SOUTH KOREA
Titolo Testata:
Journal of Endocrinology
fascicolo: 2, volume: 159, anno: 1998,
pagine: 313 - 321
SICI:
0022-0795(1998)159:2<313:IPOFAA>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELLS IN-VITRO; BOVINE GRANULOSA-CELLS; PROSTAGLANDIN PRODUCTION; CYCLIC-NUCLEOTIDES; SOMATOMEDIN-C; ESTROUS-CYCLE; OXYTOCIN; INSULIN; RELEASE; VASOPRESSIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
A.V. Sirotkin et al., "ISOLATED PORCINE OVARIAN FOLLICLES AS A MODEL FOR THE STUDY OF HORMONE AND GROWTH-FACTOR ACTION ON OVARIAN SECRETORY ACTIVITY", Journal of Endocrinology, 159(2), 1998, pp. 313-321

Abstract

The aim of our in vitro experiments with isolated porcine ovarian follicles was to study the effects of gonadotropins, GH, IGF-I and oxytocin (OT) on release of ovarian steroid, OT, IGF-I, insulin-like growth factor-binding protein-3 (IGFBP-3), prostaglandin F (PGF), prostaglandin E (PGE) and cAMP. It was found that quarters of ovarian follicles cultured for 8 days produced significant amounts of progesterone, estradiol-17 beta, OT and IGFBP-3 with peaks of accumulation from the 3rd to the 8th day of culture. Addition of serum promoted progesterone, estradiol and OT release, whilst accumulation of IGFBP-3 was maintained to a greater ex-tent in serum-free medium. GH (10 ng/ml or above) was able to inhibit androstenedione, OT, PGF and IGFBP-3, to stimulate IGF-I and cAMP, and to alter testosterone and PGE release by follicles cultured in serum-supplemented and/or serum-free medium. IGF-I (10 ng/ml or more) inhibited androstenedione and PGF secretion, stimulated testosterone, estradiol, OT and cAMP production, but did not influence progesterone, IGFBP-3 or PGE output in these conditions. OT (100 ng/ml) was able to inhibit androstenedione and to stimulate testosterone, IGF-I, PGF and PGE, but not estradiol or IGFBP-3 release. A stimulatory effect of LH on progesterone and OT and an inhibitory influence of LH on estradiol secretion in the serum-supplemented medium were observed. FSH in these conditions stimulated OT, hut not progesterone or estradiolsecretion. The use of this experimental model suggests the involvement of gonadotropins, OT, GH and IGF-I in the control of ovarian steroidand nonapeptide hormone, growth factor, growth factor-binding protein, prostaglandin and cyclic nucleotide production. The stimulatory effect of GH on IGF-I, and the stimulatory influence of IGF-I on OT, as well as coincidence of the majority of effects of IGF-I and OT, suggest the existence of a GH-IGF-I-OT axis. On the other hand, the different patterns of action of GH and IGF-I on OT, estrogen and IGFBP-3 suggestthat part of the GH effect on ovarian cells is IGF-I independent.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 21:36:42