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Titolo:
AN ADENOVIRUS VECTOR WITH GENETICALLY-MODIFIED FIBERS DEMONSTRATES EXPANDED TROPISM VIA UTILIZATION OF A COXSACKIEVIRUS AND ADENOVIRUS RECEPTOR-INDEPENDENT CELL ENTRY MECHANISM
Autore:
DMITRIEV I; KRASNYKH V; MILLER CR; WANG MH; KASHENTSEVA E; MIKHEEVA G; BELOUSOVA N; CURIEL DT;
Indirizzi:
UNIV ALABAMA,CTR COMPREHENS CANC,GENE THERAPY PROGRAM BIRMINGHAM AL 35294 UNIV ALABAMA,CTR COMPREHENS CANC,GENE THERAPY PROGRAM BIRMINGHAM AL 35294
Titolo Testata:
Journal of virology (Print)
fascicolo: 12, volume: 72, anno: 1998,
pagine: 9706 - 9713
SICI:
0022-538X(1998)72:12<9706:AAVWGF>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXTRAOVARIAN CANCER-PATIENTS; PREVIOUSLY TREATED OVARIAN; PHAGE DISPLAY LIBRARY; AMINO-ACID-SEQUENCE; ALPHA-V INTEGRINS; RECOMBINANT ADENOVIRUS; VITRONECTIN RECEPTOR; CRYSTAL-STRUCTURE; BINDING DOMAIN; VIRUS TYPE-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
I. Dmitriev et al., "AN ADENOVIRUS VECTOR WITH GENETICALLY-MODIFIED FIBERS DEMONSTRATES EXPANDED TROPISM VIA UTILIZATION OF A COXSACKIEVIRUS AND ADENOVIRUS RECEPTOR-INDEPENDENT CELL ENTRY MECHANISM", Journal of virology (Print), 72(12), 1998, pp. 9706-9713

Abstract

Recombinant adenoviruses (Ad) have become the vector system of choicefor a variety of gene therapy applications. However, the utility of Ad vectors is limited due to the low efficiency of Ad-mediated gene transfer to cells expressing marginal levels of the coxsackievirus and adenovirus receptor (CAR). In order to achieve CAR-independent gene transfer by Ad vectors in clinically important contexts, we proposed modification of viral tropism via genetic alterations to the viral fiber protein. We have shown that incorporation of an Arg-Gly-Asp (RGD)-containing peptide in the HI loop of the fiber knob domain results in the ability of the virus to utilize an alternative receptor during the cell entry process. We have also demonstrated that due to its expanded tissue tropism, this novel vector is capable of efficient transduction of primary tumor cells. An increase in gene transfer to ovarian cancer cells of 2 to 3 orders of magnitude was demonstrated by the vector, suggesting that recombinant Ad containing fibers with an incorporated RGD peptide may be of great utility for treatment of neoplasms characterized by deficiency of the primary Ad type 5 receptor.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 08:35:33