Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CONTROL OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 RNA-METABOLISM - ROLE OF SPLICE SITES AND INTRON SEQUENCES IN UNSPLICED VIRAL-RNA SUBCELLULAR-DISTRIBUTION
Autore:
SEGUIN B; STAFFA A; COCHRANE A;
Indirizzi:
UNIV TORONTO,DEPT MOL & MED GENET,MED SCI BLDG TORONTO ON M5S 1A8 CANADA UNIV TORONTO,DEPT MOL & MED GENET TORONTO ON M5S 1A8 CANADA MCGILL UNIV,DEPT MICROBIOL & IMMUNOL MONTREAL PQ CANADA
Titolo Testata:
Journal of virology (Print)
fascicolo: 12, volume: 72, anno: 1998,
pagine: 9503 - 9513
SICI:
0022-538X(1998)72:12<9503:COHTR->2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENV MESSENGER-RNA; NUCLEAR EXPORT SIGNALS; REV ACTIVATION DOMAIN; GENE-EXPRESSION; HIV REV; TRANS-ACTIVATOR; PROTEIN; IDENTIFICATION; CELLS; MATRIX;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
71
Recensione:
Indirizzi per estratti:
Citazione:
B. Seguin et al., "CONTROL OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 RNA-METABOLISM - ROLE OF SPLICE SITES AND INTRON SEQUENCES IN UNSPLICED VIRAL-RNA SUBCELLULAR-DISTRIBUTION", Journal of virology (Print), 72(12), 1998, pp. 9503-9513

Abstract

In the course of examining the various factors which affect the metabolism of human immunodeficiency virus type 1 (HIV-1) RNA, we examined the role of intron sequences and splice sites in determining the subcellular distribution of the RNA. Using in situ hybridization, we demonstrated that in the absence of Rev, unspliced RNA generated with an HIV-1 env expression construct displayed discrete localization in the nucleus, coincident with the location of the gene and not associated withSC35-containing nuclear speckles. Expression of Rev resulted in a disperse signal for the unspliced RNA throughout both the nucleus and thecytoplasm. Subsequent fractionation of the nucleus revealed that the majority of unspliced viral RNA within the nucleus is associated with the nuclear matrix and that upon expression of Rev, a small proportionof the unspliced RNA is found within the nucleoplasm. Mutations whichaltered splice site utilization did not alter the sequestration of unspliced RNA into discrete nuclear regions. In contrast, a 2.2-kb deletion of intron sequence resulted in a shift from discrete regions within the nucleus to a disperse signal throughout the cell, indicating that intron sequences, and not just splice sites, are required for the observed nuclear sequestration of unspliced viral RNA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 10:39:09