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Titolo:
DYNAMICS OF CENTROMERES DURING METAPHASE-ANAPHASE TRANSITION IN FISSION YEAST - DIS1 IS IMPLICATED IN FORCE BALANCE IN METAPHASE BIPOLAR SPINDLE
Autore:
NABESHIMA K; NAKAGAWA T; STRAIGHT AF; MURRAY A; CHIKASHIGE Y; YAMASHITA YM; HIRAOKA Y; YANAGIDA M;
Indirizzi:
KYOTO UNIV,CREST RES PROJECT,DEPT BIOPHYS,GRAD SCH SCI KYOTO 606 JAPAN KYOTO UNIV,CREST RES PROJECT,DEPT BIOPHYS,GRAD SCH SCI KYOTO 606 JAPAN UNIV CALIF SAN FRANCISCO,DEPT PHYSIOL SAN FRANCISCO CA 94143 KANSAI ADV RES CTR,COMMUN RES LAB KOBE HYOGO 65124 JAPAN
Titolo Testata:
Molecular biology of the cell
fascicolo: 11, volume: 9, anno: 1998,
pagine: 3211 - 3225
SICI:
1059-1524(1998)9:11<3211:DOCDMT>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
SISTER-CHROMATID SEPARATION; DNA TOPOISOMERASE MUTANTS; SCHIZOSACCHAROMYCES-POMBE; POLE BODY; MITOTIC SPINDLE; CHROMOSOME CONDENSATION; BUDDING YEAST; MICROTUBULE FLUX; PROTEIN; MITOSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
K. Nabeshima et al., "DYNAMICS OF CENTROMERES DURING METAPHASE-ANAPHASE TRANSITION IN FISSION YEAST - DIS1 IS IMPLICATED IN FORCE BALANCE IN METAPHASE BIPOLAR SPINDLE", Molecular biology of the cell, 9(11), 1998, pp. 3211-3225

Abstract

In higher eukaryotic cells, the spindle forms along with chromosome condensation in mitotic prophase. In metaphase, chromosomes are alignedon the spindle with sister kinetochores facing toward the opposite poles. In anaphase A, sister chromatids separate from each other withoutspindle extension, whereas spindle elongation takes place during anaphase B. We have critically examined whether such mitotic stages also occur in a lower eukaryote, Schizosaccharomyces pombe. Using the green fluorescent protein tagging technique, early mitotic to late anaphase events were observed in living fission yeast cells. S. pombe has threephases in spindle dynamics, spindle formation (phase 1), constant spindle length (phase 2), and spindle extension (phase 3). Sister centromere separation (anaphase A) rapidly occurred at the end of phase 2. The centromere showed dynamic movements throughout phase 2 as it moved back and forth and was transiently split in two before its separation, suggesting that the centromere was positioned in a bioriented manner toward the poles at metaphase. Microtubule-associating Dis1 was required for the occurrence of constant spindle length and centromere movement in phase 2. Normal transition from phase 2 to 3 needed DNA topoisomerase IT and Cut1 but not Cut14. The duration of each phase was highly dependent on temperature.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 15:57:43