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Titolo:
ALGINATE ENCAPSULATION TECHNIQUE AND CRYOGENIC PROCEDURES FOR LONG-TERM STORAGE OF THE TROPICAL FOREST TREE GUAZUMA-CRINITA MART. IN-VITRO CULTURES
Autore:
MARUYAMA E; ISHII K; KINOSHITA I;
Indirizzi:
FORESTRY & FOREST PROD RES INST,BIORESOURCES TECHNOL DIV IBARAKI OSAKA 3058687 JAPAN FORESTRY & FOREST PROD RES INST,BIORESOURCES TECHNOL DIV IBARAKI OSAKA 3058687 JAPAN
Titolo Testata:
JARQ. Japan Agricultural Research Quarterly
fascicolo: 4, volume: 32, anno: 1998,
pagine: 301 - 309
SICI:
0021-3551(1998)32:4<301:AETACP>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWN SHOOT TIPS; CRYOPRESERVATION; VITRIFICATION; MULBERRY; MERISTEMS; SURVIVAL; APPLE; BUDS; PEAR;
Keywords:
ARTIFICIAL SEED; BOLAINA BLANCA; CRYOPRESERVATION; GERMPLASM CONSERVATION; VITRIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
E. Maruyama et al., "ALGINATE ENCAPSULATION TECHNIQUE AND CRYOGENIC PROCEDURES FOR LONG-TERM STORAGE OF THE TROPICAL FOREST TREE GUAZUMA-CRINITA MART. IN-VITRO CULTURES", JARQ. Japan Agricultural Research Quarterly, 32(4), 1998, pp. 301-309

Abstract

Long-term storage of the tropical forest tree Guazuma crinita Mart. following alginate encapsulation technique and cryogenic procedures wasattempted. Shoot tips excised from in vitro plantlets were encapsulated in calcium alginate beads and stored on different substrates at 12,20, and 25 degrees C. Viability was retained when encapsulated shoot tips were stored on substrate containing only water solidified with 1%(w/v) agar. Percentage of viability 12 months after storage was 90% at 25 degrees C and 70% at 20 degrees C. For cryogenic experiments, shoot tip, nodal segment, root tip, and adventitious bud cluster explantswere stored in liquid nitrogen following 4 cryopreservation methods: (1) simple freezing, (2) rapid freezing, (3) slow pre-freezing, and (4) encapsulation/dehydration method. High survival rates (about 80%) were achieved when the adventitious bud cluster explants were exposed toa cryoprotectant mix solution containing w/v), 25% glycerol, 15% sucrose, 15% ethylene glycol, 13% dimethyl sulfoxide, and 2% polyethylene glycol (4,000), at 25 degrees C for 15-60 min prior to storage in liquid nitrogen.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 06:06:44