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Titolo:
INHIBITION OF DNA PRIMASE BY SPHINGOSINE AND ITS ANALOGS PARALLELS WITH THEIR GROWTH SUPPRESSION OF CULTURED HUMAN LEUKEMIC-CELLS
Autore:
TAMIYAKOIZUMI K; MURATE T; SUZUKI M; SIMBULAN CMG; NAKAGAWA M; TAKEMURA M; FURUTA K; IZUTA S; YOSHIDA S;
Indirizzi:
NAGOYA UNIV,SCH MED,DIS MECHANISM & CONTROL RES INST,CANC CELL BIOL LAB,SHOWA KU,TSURUMAI CHO 65 NAGOYA AICHI 466 JAPAN NAGOYA UNIV,SCH MED,DIS MECHANISM & CONTROL RES INST,CANC CELL BIOL LAB,SHOWA KU NAGOYA AICHI 466 JAPAN NAGOYA UNIV,SCH MED,DEPT INTERNAL MED 1,SHOWA KU NAGOYA AICHI 466 JAPAN CHIBA UNIV,FAC PHARMACEUT SCI,INAGE KU CHIBA 263 JAPAN
Titolo Testata:
Biochemistry and molecular biology international
fascicolo: 6, volume: 41, anno: 1997,
pagine: 1179 - 1189
SICI:
1039-9712(1997)41:6<1179:IODPBS>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; D-ERYTHRO-SPHINGOSINE; POLYMERASE-ALPHA; CALF THYMUS; HUMAN NEUTROPHILS; HL-60 CELLS; DIFFERENTIATION; TRIPHOSPHATES; BASES; DERIVATIVES;
Keywords:
SPHINGOSINE; DNA PRIMASE; DNA PRIMASE-DEPENDENT DNA SYNTHESIS; CELL GROWTH SUPPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
K. Tamiyakoizumi et al., "INHIBITION OF DNA PRIMASE BY SPHINGOSINE AND ITS ANALOGS PARALLELS WITH THEIR GROWTH SUPPRESSION OF CULTURED HUMAN LEUKEMIC-CELLS", Biochemistry and molecular biology international, 41(6), 1997, pp. 1179-1189

Abstract

Sphingosine is a potent inhibitor of a mammalian DNA primase in vitro(Simbulan et al., Biochemistry 33, 9007-9012, 1994). Here we measuredthe inhibition of DNA primase in vitro by 9 sphingosine-analogues with respect to RNA primer synthesis and DNA primase-dependent DNA synthesis, and their potencies of inhibition in vitro were compared with their in vivo effects on human leukemic cells. Sphingosine, phytosphingosine and N, N-dimethylsphingosine strongly inhibited the activity of purified calf thymus DNA primase, and also inhibited the growth of humanleukemic cell line HL-60, exerting strong cytotoxicity. Dihydrosphingosine and cis-sphingosine, which showed more subtle inhibition of DNA primase in vitro, moderately inhibited the cell growth in vivo and caused cell death. In contrast, N-acyl-, N-octyl-, and N-acetylsphingosine (ceramides) showing Little inhibition of DNA primase suppressed cellgrowth only slightly. HL 60 cell was arrested at G0/G1 phase by exogenously added sphingosine. From these results, it is suggested that DNAprimase is one of targets of sphingosine, an effector molecule in apoptosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 06:41:06