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Titolo:
DETECTION OF ENTEROVIRAL RNA IN END-STAGE DILATED CARDIOMYOPATHY IN CHILDREN AND ADOLESCENTS
Autore:
AROLA A; KALLAJOKI M; RUUSKANEN O; HYYPIA T;
Indirizzi:
TAMPERE UNIV HOSP,DEPT PEDIAT,POB 2000 FIN-33521 TAMPERE FINLAND TURKU UNIV,DEPT PEDIAT TURKU FINLAND TURKU UNIV,DEPT VIROL TURKU FINLAND TURKU UNIV,DEPT PATHOL TURKU FINLAND TURKU UNIV,MEDICITY RES LAB TURKU FINLAND UNIV HELSINKI,HAARTMAN INST,DEPT VIROL HELSINKI FINLAND
Titolo Testata:
Journal of medical virology
fascicolo: 4, volume: 56, anno: 1998,
pagine: 364 - 371
SICI:
0146-6615(1998)56:4<364:DOERIE>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; LIQUID-PHASE HYBRIDIZATION; COXSACKIEVIRUS B3 RNA; CLINICAL SPECIMENS; SEQUENCE-ANALYSIS; INSITU HYBRIDIZATION; GENE AMPLIFICATION; MYOCARDIAL TISSUE; CELL-ACTIVITY; VIRUS;
Keywords:
IDIOPATHIC DILATED CARDIOMYOPATHY; INFECTIONS; POLYMERASE CHAIN REACTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
A. Arola et al., "DETECTION OF ENTEROVIRAL RNA IN END-STAGE DILATED CARDIOMYOPATHY IN CHILDREN AND ADOLESCENTS", Journal of medical virology, 56(4), 1998, pp. 364-371

Abstract

Medical records and archival myocardial specimens of 33 children and adolescents with endstage idiopathic dilated cardiomyopathy (IDCM) were collected to evaluate retrospectively the potential role of enteroviral persistence in the pathogenesis of IDCM. The clinical history and laboratory assessment of each patient were reviewed carefully in orderto obtain information on the nature and etiology of infections in thepast and at the time of diagnosis of cardiomyopathy. Sixty-four formaldehyde-fixed, paraffin-embedded myocardial specimens, obtained from endomyocardial biopsies (n = 5), explanted hearts (n = 10), or autopsies (n = 49), were studied by the polymerase chain reaction (PCR) and byin situ hybridization to detect enteroviral RNA in the specimens. Control specimens included 34 formaldehyde-fixed, paraffin-embedded myocardial specimens from children with other cardiomyopathies, metabolic diseases, structural heart defects, or various noncardiac malignancies. The presence of cellular RNA in the specimens was confirmed by amplification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA or beta-actin mRNA as positive controls. Only one specimen from the 32 IDCMpatients with appropriate myocardial specimens was positive for enteroviral RNA by PCR. Sequence analysis of the amplified viral segment showed a significant degree of homology between the viral sequence and echovirus 1. One specimen from the control patients also appeared positive by PCR, but sequence analysis of the amplified viral segment revealed it as rhinovirus 16. The results do not indicate any significant role for enteroviral persistence in endstage childhood IDCM, although they need to be confirmed using a prospective study with fresh frozen specimens. However, mechanisms other than viral persistence may be moreimportant in the progression of IDCM to end-stage heart failure in this age group. (C) 1998 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 21:31:47