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Titolo:
PRIMER SPECIFIC AND MISPAIR EXTENSION ANALYSIS (PSMEA) AS A SIMPLE APPROACH TO FAST GENOTYPING
Autore:
HU YW; BALASKAS E; KESSLER G; ISSID C; SCULLY LJ; MURPHY DG; RINFRET A; GIULIVI A; SCALIA V; GILL P;
Indirizzi:
CANADIAN BLOOD SERV,1800 ALTA VISTA DR OTTAWA ON K1G 4JS CANADA CANADIAN RED CROSS SOC OTTAWA ON K1G 4J5 CANADA LAB SANTE PUBL QUEBEC ST ANNE BELLEVUE PQ H9X 3R5 CANADA CHUM,RES CTR MONTREAL PQ H2X 1P1 CANADA
Titolo Testata:
Nucleic acids research
fascicolo: 21, volume: 26, anno: 1998,
pagine: 5013 - 5015
SICI:
0305-1048(1998)26:21<5013:PSAMEA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEPATITIS-C VIRUS; DNA-POLYMERASE; FIDELITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
6
Recensione:
Indirizzi per estratti:
Citazione:
Y.W. Hu et al., "PRIMER SPECIFIC AND MISPAIR EXTENSION ANALYSIS (PSMEA) AS A SIMPLE APPROACH TO FAST GENOTYPING", Nucleic acids research, 26(21), 1998, pp. 5013-5015

Abstract

A simple method, primer specific and mispair extension analysis (PSMEA) with pfu DNA polymerase was developed for genotyping, PSMEA is based on the unique properties of 3'-->5' exonuclease proofreading activity, In the presence of an incomplete set of dNTPs, pfu was found to be extremely discriminative in nucleotide incorporation and proofreading at the initiation step of DNA synthesis, completely preventing primer extension when mispair(s) are found adjacent to the 3'-end of the primer. This has allowed us to accurately detect nucleotide variations, deletions and insertions for fast genotyping.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 14:56:29