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Titolo:
CLONING AND NUCLEOTIDE-SEQUENCE OF THE ALCOHOL ACETYLTRANSFERASE II GENE (ATF2) FROM SACCHAROMYCES-CEREVISIAE KYOKAI NO. 7
Autore:
NAGASAWA N; BOGAKI T; IWAMATSU A; HAMACHI M; KUMAGAI C;
Indirizzi:
OZEKI CORP,GEN RES LAB,KANAZAWA KU,4-9 IMAZU DEZAIKE CHO NISHINOMIYA HYOGO 6638227 JAPAN
Titolo Testata:
Bioscience, biotechnology, and biochemistry
fascicolo: 10, volume: 62, anno: 1998,
pagine: 1852 - 1857
SICI:
0916-8451(1998)62:10<1852:CANOTA>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESTER FORMATION; BREWERS-YEAST; MUTANTS; EXPRESSION; ACETATE;
Keywords:
SACCHAROMYCES CEREVISIAE; ALCOHOL ACETYLTRANSFERASE; ATF2; GENE CLONING; ISOAMYL ACETATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
N. Nagasawa et al., "CLONING AND NUCLEOTIDE-SEQUENCE OF THE ALCOHOL ACETYLTRANSFERASE II GENE (ATF2) FROM SACCHAROMYCES-CEREVISIAE KYOKAI NO. 7", Bioscience, biotechnology, and biochemistry, 62(10), 1998, pp. 1852-1857

Abstract

The ATF2 gene, which encodes alcohol acetyltransferase II. (AATase II), was cloned from Saccharomyces cerevisiae Kyokai No. 7 (sake yeast). The ATF2 gene coded for a protein of 535 amino acid residues with a calculated molecular mass of 61,909 daltons. The deduced amino acid sequences of the ATF2 showed 36.9% similarity with that of ATF1, which encodes AATase I. The hydrophobicity profiles for the Atf2 protein and Atf1 protein were similar. A transformant carrying multiple copies of the ATF2 gene had 2.5-fold greater AATase activity than the control, and this activity was not significantly inhibited by linoleic acid. A Southern analysis of the yeast genomes in which the ATF2 gene was used as a probe showed that S. cerevisiae and brewery lager yeast have one ATET gene, while S, bayanus has no similar gene.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 03:01:42