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Titolo:
ROLE OF 2 SERIES OF CA2+ OSCILLATIONS IN ACTIVATION OF ASCIDIAN EGGS
Autore:
YOSHIDA M; SENSUI N; INOUE T; MORISAWA M; MIKOSHIBA K;
Indirizzi:
UNIV TOKYO,GRAD SCH SCI,MISAKI MARINE BIOL STN,1024 KAOJIRO KANAGAWA 2380225 JAPAN RIKEN,INST PHYS & CHEM RES,TSUKUBA LIFE SCI CTR,MOL NEUROBIOL LAB TSUKUBA IBARAKI 3050074 JAPAN UNIV TOKYO,GRAD SCH SCI,MISAKI MARINE BIOL STN KANAGAWA 2380225 JAPAN UNIV RYUKYUS,FAC MED,DEPT ANAT NISHIHARA OKINAWA 9030215 JAPAN UNIV TOKYO,INST MED SCI,DEPT MOL NEUROBIOL TOKYO 1088639 JAPAN JAPAN SCI & TECHNOL CORP,ERATO,MIKOSHIBA CALCIOSIGNAL NET PROJECT TOKYO 1530064 JAPAN
Titolo Testata:
Developmental biology (Print)
fascicolo: 1, volume: 203, anno: 1998,
pagine: 122 - 133
SICI:
0012-1606(1998)203:1<122:RO2SOC>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR; SEA-URCHIN EGGS; CALCIUM WAVES; CELL-CYCLE; OOPLASMIC SEGREGATION; PENICILLIUM-BREVICOMPACTUM; CIONA-INTESTINALIS; MEIOTIC MATURATION; POTENT AGONISTS; MAMMALIAN EGGS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
65
Recensione:
Indirizzi per estratti:
Citazione:
M. Yoshida et al., "ROLE OF 2 SERIES OF CA2+ OSCILLATIONS IN ACTIVATION OF ASCIDIAN EGGS", Developmental biology (Print), 203(1), 1998, pp. 122-133

Abstract

Changes in [Ca2+](i) are an essential factor regulating egg activation. Matured ascidian eggs are arrested at metaphase I, and two series of [Ca2+](i) transients have been observed after fertilization: Ca2+ waves just after fertilization (Series I) and [Ca2+](i) oscillation between the first and second polar body extrusion (Series II). We investigated mechanisms involved in the elevation of [Ca2+](i) and the role ofthe [Ca2+](i) transients during egg activation in Ciona savignyi. Themonoclonal antibody 18A10 against IP3 receptor type 1, which inhibitsIP3-induced Ca2+ release in hamster and mouse eggs, did not show substantial inhibitory effects on series I or egg deformation, whereas Series II and the first cell division were inhibited by the antibody. Ruthenium red, an inhibitor of ryanodine receptor-mediated Ca2+ release, had no apparent effect of [Ca2+](i) transients and other events related to the egg activation. Microinjection of IP3 into unfertilized eggs induced [Ca2+](i) transients similar to those seen in Series I, whereas injection of cyclic ADP ribose, an agonist of ryanodine receptors, rarely induced [Ca2+](i) transient. Adenophostin B, a potent nonmetabolizable agonist of IP3 receptors, induced [Ca2+](i) oscillations which continued after first polar body extrusion, without separation to two series, and led to extrusion of first and second polar bodies. These results suggest that Series II is driven by the mouse type 1-like IP3 receptor while Series I seems to be mediated by another type of IP3 receptor. Injection of IP3 only induced the first polar body extrusion and the egg was arrested at metaphase II even when a higher amount of IP3 was injected. On the other hand, reinjection of IP3 after the first polar body extrusion led to emission of the second polar body. Thus, Series I and II of [Ca2+](i) transients are likely to be required for metaphase-anaphase transition in meiosis. (C) 1998 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 23:36:24