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Titolo:
2-DIMENSIONAL MASS-SPECTROMETRY OF BIOMOLECULES AT THE SUBFEMTOMOLE LEVEL
Autore:
MCLAFFERTY FW; KELLEHER NL; BEGLEY TP; FRIDRIKSSON EK; ZUBAREV RA; HORN DM;
Indirizzi:
CORNELL UNIV,BAKER LAB CHEM ITHACA NY 14853
Titolo Testata:
Current opinion in chemical biology
fascicolo: 5, volume: 2, anno: 1998,
pagine: 571 - 578
SICI:
1367-5931(1998)2:5<571:2MOBAT>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
MULTIPLY-CHARGED IONS; INFRARED MULTIPHOTON DISSOCIATION; ELECTROSPRAY-IONIZATION; LARGE MOLECULES; SEQUENCE INFORMATION; PROTEINS; ACCURACY; IDENTIFICATION; COMPLEXES; PEPTIDES;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
F.W. Mclafferty et al., "2-DIMENSIONAL MASS-SPECTROMETRY OF BIOMOLECULES AT THE SUBFEMTOMOLE LEVEL", Current opinion in chemical biology, 2(5), 1998, pp. 571-578

Abstract

Multiple dimensions of unique molecular structure information can nowbe obtained from proteins and DNA using mass spectrometry. Less than 10(-16) mol of the active major histocompatibility complex signaling peptide in a mixture of thousands can be identified. For large proteins(>40 kDa), the high resolving power (>10(5)) and 10(-17) mol sensitivity of Fourier-transform mass spectrometry provide exact molecular weight values (+/- 1 or 2 Da) for mixture components, indicating errors or modifications compared with the predicted DNA sequence. Selecting a specific molecular species, its two-dimensional spectrum indicates thepart of the molecule that is modified; a three-dimensional spectrum of that fragment further isolates the modification site.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 05:57:20