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Titolo:
STH1P, A SACCHAROMYCES-CEREVISIAE SNF2P SWI2P HOMOLOG, IS AN ESSENTIAL ATPASE IN RSC AND DIFFERS FROM SNF/SWI IN ITS INTERACTIONS WITH HISTONES AND CHROMATIN-ASSOCIATED PROTEINS/
Autore:
DU J; NASIR I; BENTON BK; KLADDE MP; LAURENT BC;
Indirizzi:
SUNY HLTH SCI CTR,MORSE INST MOL GENET,450 CLARKSON AVE,BOX 44 BROOKLYN NY 11203 SUNY HLTH SCI CTR,MORSE INST MOL GENET BROOKLYN NY 11203 SUNY HLTH SCI CTR,DEPT MICROBIOL & IMMUNOL BROOKLYN NY 11203 ROCKEFELLER UNIV NEW YORK NY 10021 PENN STATE UNIV,DEPT BIOCHEM & MOL BIOL UNIVERSITY PK PA 16802
Titolo Testata:
Genetics
fascicolo: 3, volume: 150, anno: 1998,
pagine: 987 - 1005
SICI:
0016-6731(1998)150:3<987:SASSSH>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-CYCLE ARREST; UPSTREAM ACTIVATING SEQUENCE; NUCLEOSOME REMODELING FACTOR; YEAST SWI/SNF COMPLEX; SWI-SNF COMPLEX; BUDDING YEAST; TRANSCRIPTIONAL ACTIVATORS; BINDING PROTEIN; IN-VIVO; MULTISUBUNIT COMPLEX;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
105
Recensione:
Indirizzi per estratti:
Citazione:
J. Du et al., "STH1P, A SACCHAROMYCES-CEREVISIAE SNF2P SWI2P HOMOLOG, IS AN ESSENTIAL ATPASE IN RSC AND DIFFERS FROM SNF/SWI IN ITS INTERACTIONS WITH HISTONES AND CHROMATIN-ASSOCIATED PROTEINS/", Genetics, 150(3), 1998, pp. 987-1005

Abstract

The essential Sth1p is the protein most closely related to the conserved Snf2p/Swi2p in Saccharomyces cerevisiae. Sth1p purified from yeasthas a DNA-stimulated ATPase activity required for its function in vivo. The finding that Sth1p is a component of a multiprotein complex capable of ATP-dependent remodeling of the structure of chromatin (RSC) in vitro, suggests that it provides RSC with ATP hydrolysis activity. Three sth1 temperature-sensitive mutations map to the highly conserved ATPase/helicase domain and have cell cycle and non-cell cycle phenotypes, suggesting multiple essential roles for Sth1p. The Sth1p bromodomain is required for wild-type function; deletion mutants lacking portions of this region are thermosensitive and arrest with highly elongatedbuds and 2C DNA content, indicating perturbation of a unique function. The pleiotropic growth defects of sth1-ts mutants imply a requirement for Sthlp in a general cellular process that affects several metabolic pathways. Significantly, an sth1-ts allele is synthetically sick orlethal with previously identified mutations in histones and chromatinassembly genes that suppress snf/swi, suggesting that RSC interacts differently with chromatin than Snf/Swi. These results provide a framework for understanding the ATP-dependent RSC function in modeling chromatin and its connection to the cell cycle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 12:08:13