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Titolo:
EFFECT OF A RECOMBINANT LECTIN, VISCUM-ALBUM AGGLUTININ ON THE SECRETION OF INTERLEUKIN-12 IN CULTURED HUMAN PERIPHERAL-BLOOD MONONUCLEAR-CELLS AND ON NK-CELL-MEDIATED CYTOTOXICITY OF RAT SPLENOCYTES IN-VITRO AND IN-VIVO
Autore:
HAJTO T; HOSTANSKA K; WEBER K; ZINKE H; FISCHER J; MENGS U; LENTZEN H; SALLER R;
Indirizzi:
POB 37 CH-4132 MUTTENZ 2 SWITZERLAND UNIV ZURICH HOSP,DEPT INTERNAL MED ZURICH SWITZERLAND RES & CONSULTING CO ITTINGEN SWITZERLAND BIOTECHNOL RES & INFORMAT NETWORK GMBH ZWINGENBERG GERMANY MADAUS AG,DEPT RES COLOGNE GERMANY ALBERT SZENT GYORGYI MED UNIV,DEPT BIOCHEM H-6701 SZEGED HUNGARY
Titolo Testata:
Natural immunity
fascicolo: 1, volume: 16, anno: 1998,
pagine: 34 - 46
SICI:
1018-8916(1998)16:1<34:EOARLV>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
GALACTOSIDE-SPECIFIC LECTIN; NATURAL-KILLER-CELLS; NECROSIS-FACTOR-ALPHA; STIMULATORY FACTOR; MISTLETOE EXTRACT; GENE-EXPRESSION; TARGET-CELLS; IL-12; MOLECULES; RECEPTOR;
Keywords:
RECOMBINANT MISTLETOE LECTIN; IL-12; NK CELL ACTIVITY; MAC-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
T. Hajto et al., "EFFECT OF A RECOMBINANT LECTIN, VISCUM-ALBUM AGGLUTININ ON THE SECRETION OF INTERLEUKIN-12 IN CULTURED HUMAN PERIPHERAL-BLOOD MONONUCLEAR-CELLS AND ON NK-CELL-MEDIATED CYTOTOXICITY OF RAT SPLENOCYTES IN-VITRO AND IN-VIVO", Natural immunity, 16(1), 1998, pp. 34-46

Abstract

A plant lectin, Viscum album agglutinin-I (VAA-I) has been shown to increase the number and cytotoxic activity of natural killer (NK) cellsin animal models, but the mechanisms underlying these effects are poorly understood. We investigated the effects of the recombinant form ofthis lectin (rVAA) on secretion of interleukin (IL)-12 and on NK-mediated cytotoxicity against K562 target cells in cultures of human peripheral blood mononuclear cells (PBMC) as well as against YAC-1 target cells in cultured rat spleen cells. In 24-hour cultures of PBMC, 10 ng/ml plant VAA-I and 50 ng/ml rVAA induced significant increases in the secretion of total IL-12. Its biologically active heterodimeric form, p70, was also significantly induced by rVAA. Preincubation of PBMC or splenocytes for 48 h with rVAA in concentrations ranging between 10 pg/ml and 100 pg/ml resulted in moderate enhancements of NK-mediated cytotoxicity. However, coincubation of a low dose of rVAA (100 pg/ml) together with IL-2 and IL-12 (60 U/ml and 2 U/ml, respectively) led to additive stimulation of NK activity. In in vivo experiments, rVAA showedan enhancing effect on NK activity with a bell-shaped curve of efficacy. Forty-eight hours after a single intravenous injection of its mosteffective doses, 0.5 and 1 ng/kg, into Wistar rats, the NK cytotoxicity of splenocytes against YAC-1 targets doubled, and the frequency of large granular lymphocytes in peripheral blood showed 2.1- and 3-fold increases as compared to control animals. Twenty-four hours following these low lectin doses, the number of large granular lymphocytes was also significantly elevated. After 48 h? 0.5 ng/kg rVAA induced a significant augmentation in the percentage of peripheral Mac-it mononuclearcells, including activated monocytes and NK cells. The present results suggest that rVAA augments the secretion of an active form of IL-12 and potentiates the cytokine-induced NK activation. These effects of rVAA may be related to its stimulatory effects on MHC-unrestricted cytotoxicity in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 12:33:46