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Titolo:
EVALUATION OF A PCR KIT FOR THE DETECTION OF ERWINIA-CAROTOVORA SUBSPATROSEPTICA ON POTATO-TUBERS
Autore:
FRECHON D; EXBRAYAT P; HELIAS V; HYMAN LJ; JOUAN B; LLOP P; LOPEZ MM; PAYET N; PEROMBELON MCM; TOTH IK; VANBECKHOVEN JRCM; VANDERWOLF JM; BERTHEAU Y;
Indirizzi:
INST NATL AGRON PARIS GRIGNON,INST NATL RECH AGRON,16 RUE CLAUDE BERNARD F-75231 PARIS FRANCE INST NATL RECH AGRON F-35653 LE RHEU FRANCE SCOTTISH CROP RES INST DUNDEE DD2 5DA SCOTLAND INST VALENCIANO INVEST AGR VALENCIA 46113 SPAIN DLO,INST PLANTEZIEKTENDUNDIG ONDERZOEK NL-6700 GW WAGENINGEN NETHERLANDS INST NATL AGRON PARIS GRIGNON,INST NATL RECH AGRON F-75231 PARIS FRANCE
Titolo Testata:
Potato research
fascicolo: 2, volume: 41, anno: 1998,
pagine: 163 - 173
SICI:
0014-3065(1998)41:2<163:EOAPKF>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCLONAL-ANTIBODIES; DETECTION SENSITIVITY; SEED;
Keywords:
BLACKLEG; ELISA; DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
D. Frechon et al., "EVALUATION OF A PCR KIT FOR THE DETECTION OF ERWINIA-CAROTOVORA SUBSPATROSEPTICA ON POTATO-TUBERS", Potato research, 41(2), 1998, pp. 163-173

Abstract

A PCR-based kit, Probelia(TM), for the detection of Erwinia carotovora subsp. atroseptica (Eca) on potatoes was evaluated at five laboratories in four countries. The kit is based on DNA-specific PCR amplification followed by detection of amplicons by hybridization to a peroxidase-labelled DNA probe in a microplate. Specificity of the PCR primers for Eca, regardless of serogroups, was confirmed by testing against 246bacterial, fungal and plant species. Detection limits of the assay varied little between six Eca strains in pure cultures (1.3x10(2) to 1.5x10(3) cells ml(-1)). When Eca-free tuber peel extract from four cultivars was inoculated with known numbers of 15 Eca strains, detection Limits were more variable (1.0x10(1) to 6.2x10(3) cells ml(-1) peel extract), attributed probably to inconsistency in the recovery of DNA during extraction. When the PCR assay was compared with three current commercial Eca detection methods, using naturally contaminated tubers, results matched most closely those from viable counts on a selective medium, the most sensitive method (88%), followed by enrichment ELISA (72%) and last ELISA (30%), the least sensitive method.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 05:00:44