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Titolo:
INSULIN-REGULATED PROTEIN-KINASES DURING POSTNATAL-DEVELOPMENT OF RAT-HEART
Autore:
KIM SO; HASHAM MI; KATZ S; PELECH SL;
Indirizzi:
1779 W 75TH AVE VANCOUVER BC V6P 6P2 CANADA UNIV BRITISH COLUMBIA,FAC PHARMACEUT SCI VANCOUVER BC V6T 123 CANADA UNIV BRITISH COLUMBIA,DEPT MED VANCOUVER BC V6T 123 CANADA
Titolo Testata:
Journal of cellular biochemistry
fascicolo: 3, volume: 71, anno: 1998,
pagine: 328 - 339
SICI:
0730-2312(1998)71:3<328:IPDPOR>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
FATTY-ACID OXIDATION; STIMULATED GLUCOSE-TRANSPORT; CELLULAR ENERGY METABOLISM; 3T3-L1 ADIPOCYTES; GLYCOGEN-SYNTHASE; FETAL DEVELOPMENT; ACTIVATION; TRANSLOCATION; SUFFICIENT; ALPHA;
Keywords:
INSULIN; HEART; DEVELOPMENT; PI 3-KINASE; PROTEIN KINASE B; S6 KINASE; CASEIN KINASE 2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
S.O. Kim et al., "INSULIN-REGULATED PROTEIN-KINASES DURING POSTNATAL-DEVELOPMENT OF RAT-HEART", Journal of cellular biochemistry, 71(3), 1998, pp. 328-339

Abstract

The control of glucose uptake and glycogen metabolism by insulin in target organs is in part mediated through the regulation of protein-serine/threonine kinases. In this study, the expression and phosphotransferase activity levels of some of these kinases in rat heart ventricle were measured to investigate whether they might mediate the sh ift in the energy dependency of the developing heart from glycogen to fatty acids. Following tail-vein injection of overnight fasted adult rats with 2 U of insulin per kg body weight, protein kinase B (PKB), the 70-kDa ribosomal S6 kinase (S6K), and casein kinase 2 (CK2) were activated (30-600%), whereas the MAP/extracellular regulated kinases (ERK)1 and ERK2 were not stimulated under these conditions. When the expression levels of the insulin-activated kinases were probed with specific antibodies in ventricular extracts from 1-, 10-, 20-, 50-,and 365-day-old rats, phosphatidylinositol 3-kinase (PI3K), PKB, S6K, and CK2 were downregulated (40-60%) with age. By contrast, ventricular glycogen synthase kinase-3 beta (GSK3 beta) protein levels were maintained during postnatal development. Similar findings were obtained when the expression of these kinases was investigated in freshly isolated ventricular myocytes, where they were detected predominantly in the cytosolic fractionof the myocytes. Compared to other adult rat tissues such as brain and liver, the levels of P13K, PKB, S6K, and GSK3 beta were relatively low in the heart. Even though CK2 protein and activity levels were reduced by similar to 60% in 365 day as compared to 1-day-old rats, expression of CK2 in the adult heart was as high as detected in any of the other rat tissues. The high basal activities of CK2 in early neonatal heart may be associated with the proliferating state of myocytes. J. Cell. Biochem. 71:328-339, 1998. (C) 1998 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 11:44:04