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Titolo:
COLLAGEN IN TISSUE-ENGINEERED CARTILAGE - TYPES, STRUCTURE, AND CROSS-LINKS
Autore:
RIESLE J; HOLLANDER AP; LANGER R; FREED LE; VUNJAKNOVAKOVIC G;
Indirizzi:
MIT,DIV HLTH SCI & TECHNOL,E25-342,45 CARLETON ST CAMBRIDGE MA 02139 MIT,DIV HLTH SCI & TECHNOL CAMBRIDGE MA 02139 UNIV SHEFFIELD,DEPT HUMAN METAB & CLIN BIOCHEM SHEFFIELD S10 2RX S YORKSHIRE ENGLAND
Titolo Testata:
Journal of cellular biochemistry
fascicolo: 3, volume: 71, anno: 1998,
pagine: 313 - 327
SICI:
0730-2312(1998)71:3<313:CITC-T>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; ARTICULAR-CARTILAGE; II COLLAGEN; IX COLLAGEN; EXTRACELLULAR-MATRIX; HYDROXYPYRIDINIUM CROSSLINKS; MONOCLONAL-ANTIBODIES; QUANTITATIVE-ANALYSIS; FORMATION INVITRO; ALGINATE BEADS;
Keywords:
ARTICULAR CARTILAGE REPAIR; TISSUE ENGINEERING; COLLAGEN TYPE II; COLLAGEN TYPE IX; COLLAGEN NETWORK; PYRIDINIUM CROSS-LINKS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
J. Riesle et al., "COLLAGEN IN TISSUE-ENGINEERED CARTILAGE - TYPES, STRUCTURE, AND CROSS-LINKS", Journal of cellular biochemistry, 71(3), 1998, pp. 313-327

Abstract

The function of articular cartilage as a weight-bearing tissue depends on the specific arrangement of collagen types II and IX into a three-dimensional organized collagen network that can balance the swelling pressure of the proteoglycan/water gel. To determine whether cartilageengineered in vitro contains a functional collagen network, chondrocyte-polymer constructs were cultured for up to 6 weeks and analyzed with respect to the composition and ultrastructure of collagen by using biochemical and immunochemical methods and scanning electron microscopy. Total collagen content and the concentration of pyridinium crosslinks were significantly (57% and 70%, respectively) lower in tissue-engineered cartilage that in bovine calf articular cartilage. However, the fractions of collagen types II, IX, and X and the collagen network organization, density, and fibril diameter in engineered cartilage were not significantly different from those in natural articular cartilage. The implications of these findings for the field of tissue engineeringare that differentiated chondrocytes are capable of forming a complexstructure of collagen matrix in vitro, producing a tissue similar to natural articular cartilage on an ultrastructural scale. I. Cell. Biochem. 71 :313-327, 1998. (C) 1998 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 07:58:16