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Titolo:
FPP MODULATES MAMMALIAN SPERM FUNCTION VIA TCP-11 AND THE ADENYLYL-CYCLASE CAMP PATHWAY
Autore:
ADEOYAOSIGUWA SA; DUDLEY RK; HOSSEINI R; FRASER LR;
Indirizzi:
UNIV LONDON KINGS COLL LONDON WC2R 2LS ENGLAND UNIV LONDON KINGS COLL LONDON WC2R 2LS ENGLAND UNIV LONDON KINGS COLL,RANDALL INST,DEV BIOL RES CTR LONDON ENGLAND
Titolo Testata:
Molecular reproduction and development
fascicolo: 4, volume: 51, anno: 1998,
pagine: 468 - 476
SICI:
1040-452X(1998)51:4<468:FMMSFV>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
FERTILIZATION-PROMOTING PEPTIDE; PROTEIN-TYROSINE PHOSPHORYLATION; THYROTROPIN-RELEASING-HORMONE; TRH-RELATED TRIPEPTIDE; MOUSE SPERM; IN-VITRO; CAPACITATION INVITRO; ACROSOME REACTION; SEMINAL FLUID; SPERMATOZOA;
Keywords:
ADENOSINE; CAPACITATION; FERTILIZATION PROMOTING; PEPTIDE; T-COMPLEX;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
S.A. Adeoyaosiguwa et al., "FPP MODULATES MAMMALIAN SPERM FUNCTION VIA TCP-11 AND THE ADENYLYL-CYCLASE CAMP PATHWAY", Molecular reproduction and development, 51(4), 1998, pp. 468-476

Abstract

Fertilization promoting peptide (FPP; pGlu-Glu-ProNH(2)), which is found in seminal plasma, promotes capacitation but inhibits spontaneous acrosome loss in mammalian spermatozoa in vitro. Adenosine, known to modulate the adenylyl cyclase (AC)/cAMP pathway, elicits these same responses whereas FPP + adenosine produces an enhanced response, leading to the hypothesis that FPP and adenosine modulate the same signal transduction pathway but act via different receptors. TCP-11, the product of a t-complex gene, is the putative receptor for FPP: Fab fragments of anti-TCP-11 antibodies have the same effect as FPP on mouse spermatozoa and Gln-FPP, a competitive inhibitor of FPP, also competitively inhibits responses to the Fab fragments. In the present study, specific binding of H-3-FPP to sperm membranes was significantly inhibited by 200 nM Gln-FPP and anti-TCP-11 Fab fragments (1/25 dilution), thus confirming that FPP, Gln-FPP, and Fab fragments compete for the same binding site. In addition, spermatozoa treated with A23187 to induce the acrosome reaction bound significantly less H-3-FPP than untreated cells,suggesting that a large proportion of the FPP binding sites are associated with the acrosomal cap region; TCP-11 is located in this region. In other experiments, 100 nM FPP significantly stimulated cAMP production in mouse sperm membranes, permeabilized cells and intact cells. Furthermore, Gln-FPP inhibited production of cAMP in response to FPP but not to adenosine (10 mu M) or its analogue NECA (100 nM), supportingthe involvement of two different receptors. Finally, anti-TCP-Il Fab fragments (1/25 dilution) significantly stimulated cAMP production, whereas low Fab (1/200; nonstimulatory when used alone) plus adenosine (10 mu M) significantly enhanced the stimulation of capacitation by adenosine. These results support the hypotheses that TCP-11 is the receptor for FPP and that FPP<-->TCP-11 interactions modulate AC/cAMP. Mol. Reprod. Dev. 51:468-476, 1998. (C) 1998 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 02:27:25