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Titolo:
PATCH-CLAMP AND AMPEROMETRIC RECORDINGS FROM NOREPINEPHRINE TRANSPORTERS - CHANNEL ACTIVITY AND VOLTAGE-DEPENDENT UPTAKE
Autore:
GALLI A; BLAKELY RD; DEFELICE LJ;
Indirizzi:
VANDERBILT UNIV,MED CTR,DEPT PHARMACOL NASHVILLE TN 37232 VANDERBILT UNIV,MED CTR,DEPT PHARMACOL NASHVILLE TN 37232 VANDERBILT UNIV,MED CTR,CTR MOL NEUROSCI NASHVILLE TN 37232
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 22, volume: 95, anno: 1998,
pagine: 13260 - 13265
SICI:
0027-8424(1998)95:22<13260:PAARFN>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN DOPAMINE TRANSPORTER; SEROTONIN TRANSPORTERS; CHROMAFFIN CELLS; NEUROTRANSMITTER TRANSPORTERS; PHEOCHROMOCYTOMA CELLS; VESICLE FUSION; HEK-293 CELLS; PC-12 CELLS; NORADRENALINE; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
A. Galli et al., "PATCH-CLAMP AND AMPEROMETRIC RECORDINGS FROM NOREPINEPHRINE TRANSPORTERS - CHANNEL ACTIVITY AND VOLTAGE-DEPENDENT UPTAKE", Proceedings of the National Academy of Sciences of the United Statesof America, 95(22), 1998, pp. 13260-13265

Abstract

Transporters for the biogenic amines dopamine, norepinephrine, epinephrine and serotonin are largely responsible for transmitter inactivation after release. They also serve as high-affinity targets for a number of clinically relevant psychoactive agents, including antidepressants, cocaine, and amphetamines. Despite their prominent role in neurotransmitter inactivation and drug responses, we lack a clear understanding of the permeation pathway or regulation mechanisms at the single transporter level. The resolution of radiotracer-based flux techniques limits the opportunities to dissect these problems, Here we combine patch-clamp recording techniques with micro-amperometry to record the transporter-mediated flux of norepinephrine across isolated membrane patches. These data reveal voltage-dependent norepinephrine flux that correlates temporally with antidepressant-sensitive transporter currents inthe same patch. Furthermore, we resolve unitary flux events linked with bursts of transporter channel openings. These findings indicate that norepinephrine transporters are capable of transporting neurotransmitter across the membrane in discrete shots containing hundreds of molecules, Amperometry is used widely to study neurotransmitter distribution and kinetics in the nervous system and to detect transmitter release during vesicular exocytosis. Of interest regarding the present application is the use of amperometry on inside-out patches with synchronous recording of flux and current. Thus, our results further demonstratea powerful method to assess transporter function and regulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 12:24:29