Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
HTERT IS A CRITICAL DETERMINANT OF TELOMERASE ACTIVITY IN RENAL-CELL CARCINOMA
Autore:
KANAYA T; KYO S; TAKAKURA M; ITO H; NAMIKI M; INOUE M;
Indirizzi:
KANAZAWA UNIV,DEPT GYNECOL & OBSTET,SCH MED,13-1 TAKARAMACHI KANAZAWAISHIKAWA 920093 JAPAN KANAZAWA UNIV,DEPT GYNECOL & OBSTET,SCH MED KANAZAWA ISHIKAWA 920093 JAPAN KANAZAWA UNIV,SCH MED,DEPT UROL KANAZAWA ISHIKAWA 920 JAPAN
Titolo Testata:
International journal of cancer
fascicolo: 5, volume: 78, anno: 1998,
pagine: 539 - 543
SICI:
0020-7136(1998)78:5<539:HIACDO>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAMMALIAN TELOMERASE; RNA COMPONENT; PROTEIN; TUMORS; CANCER; GENE; DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
T. Kanaya et al., "HTERT IS A CRITICAL DETERMINANT OF TELOMERASE ACTIVITY IN RENAL-CELL CARCINOMA", International journal of cancer, 78(5), 1998, pp. 539-543

Abstract

Telomerase is a ribonucleoprotein enzyme which stabilizes chromosomalstructure, thereby inducing cellular immortality. Three major subunits composing telomerase complex have been cloned, designated hTR (humantelomerase RNA), TPI (telomerase-associated protein I), and hTERT (human telomerase reverse transcriptase). In the present study, a total of 36 renal-cell carcinomas(RCC) and adjacent normal tissues, as well as cell lines derived from RCC or normal kidney, were examined for the expression of each telomerase subunit and telomerase activity. RT-PCR analyses revealed that hTR and TPI mRNA were constitutively expressed both in tumor and in normal tissues. In contrast, hTERT mRNA was expressed in most tumors, but not in normal tissues. Telomeric-repeat-amplification-protocol (TRAP) assay revealed that more than 80% of RCC tumor tissue exhibited telomerase activity, while none of the adjacent normal tissue did. There was a significant association of telomerase activity with expression of hTERT mRNA, but not with TP I mRNA or hTR expression. Two cell lines, derived from RCC and cervical cancer, expressed telomerase activity and hTERT mRNA, while normal renal cortical epithelial cells expressed neither of them. These findings suggest that hTERT plays a critical role in determining the enzymatic activity of human telomerase, and that up-regulation of hTERT probably plays a role in the progression of human cancers. (C) 1998 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 20:48:40