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Titolo:
SEASONAL DIFFERENCES IN ACTIVITY OF PERCH (PERCA-FLAVESCENS) GILL NA+K+ ATPASE/
Autore:
PACKER RK; GARVIN JL;
Indirizzi:
GEORGE WASHINGTON UNIV,DEPT BIOL SCI WASHINGTON DC 20052 HENRY FORD HOSP,DEPT MED,HYPERTENS & VASC RES DIV DETROIT MI 48202
Titolo Testata:
Comparative biochemistry and physiology. B. Comparative biochemistry
fascicolo: 4, volume: 120, anno: 1998,
pagine: 777 - 783
SICI:
0305-0491(1998)120:4<777:SDIAOP>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADENOSINE-TRIPHOSPHATASE; SALVELINUS-ALPINUS; ANGUILLA-ANGUILLA; TEMPERATURE; WATER; ACCLIMATION; FISH; OSMOREGULATION; DEHYDROGENASE; MEMBRANES;
Keywords:
NA+/K+ ATPASE; GILL; FISH; ACCLIMATIZATION; TEMPERATURE; ION BALANCE; SEASONAL ENZYMES; V-MAX; K-M;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
R.K. Packer e J.L. Garvin, "SEASONAL DIFFERENCES IN ACTIVITY OF PERCH (PERCA-FLAVESCENS) GILL NA+K+ ATPASE/", Comparative biochemistry and physiology. B. Comparative biochemistry, 120(4), 1998, pp. 777-783

Abstract

We measured Na+/K+ ATPase activity in homogenates of gill tissue prepared from field caught, winter and summer acclimatized yellow perch, Perca flavescens. Water temperatures were 2-4 degrees C in winter and 19-22 degrees C in summer. Na+/K+ ATPase activity was measured at 8, 17, 25, and 37 degrees C. V-max values for winter fish increased from 0.48 +/- 0.07 mu mol P mg(-1) protein h(-1) at 8 degrees C to 7.21 +/- 0.79 mu mol P mg(-1) protein h(-1) at 37 degrees C. In summer fish it ranged from 0.46 +/- 0.08 (8 degrees C) to 3.86 +/- 0.50 (37 degrees C)mu mol P mg(-1) protein h(-1). The K-m for ATP and for Na+ at 8 degrees C was approximate to 1.6 and 10 mM, respectively and did not vary significantly with assay temperature in homogenates from summer fish. The activation energy for Na+/K+ ATPase from summer fish was 10309 (mu mol P mg(-1) h(-1)) K-1. In winter fish, the K-m for ATP and Na+ increased from 0.59 +/- 0.08 mM and 9.56 +/- 1.18 mM at 8 degrees C to 1.49+/- 0.11 and 17.88 +/- 2.64 mM at 17 degrees C. The K-m values for ATP and Na did not vary from 17 to 37 degrees C. A single activation energy could not be calculated for:Na/K ATPase from winter fish. The observed differences in enzyme activities and affinities could be due to seasonal changes in membrane lipids, differences in the amount of enzyme, or changes in isozyme expression. (C) 1998 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 08:26:54