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Titolo:
EXPRESSION AND LOCALIZATION OF MEMBERS OF THE FIBROBLAST-GROWTH-FACTOR FAMILY IN THE BOVINE MAMMARY-GLAND
Autore:
PLATH A; EINSPANIER R; GABLER C; PETERS F; SINOWATZ F; GOSPODAROWICZ D; SCHAMS D;
Indirizzi:
TECH UNIV MUNCHEN WEIHENSTEPHAN,INST PHYSIOL D-85350 FREISING GERMANY ST HILDEGARDIS HOSP D-55131 MAINZ GERMANY UNIV MUNICH D-80539 MUNICH GERMANY CHIRON CORP EMERYVILLE CA 94608
Titolo Testata:
Journal of dairy science
fascicolo: 10, volume: 81, anno: 1998,
pagine: 2604 - 2613
SICI:
0022-0302(1998)81:10<2604:EALOMO>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
MYOEPITHELIAL-LIKE CELLS; ENDOTHELIAL-CELLS; FACTOR RECEPTORS; MESSENGER-RNAS; IN-VIVO; KERATINOCYTE; DIFFERENTIATION; CULTURE; BINDING; EPITHELIUM;
Keywords:
BOVINE; MAMMARY GLAND; GENE EXPRESSION; FIBROBLAST GROWTH FACTORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
A. Plath et al., "EXPRESSION AND LOCALIZATION OF MEMBERS OF THE FIBROBLAST-GROWTH-FACTOR FAMILY IN THE BOVINE MAMMARY-GLAND", Journal of dairy science, 81(10), 1998, pp. 2604-2613

Abstract

The goal of the study was to examine the expression and localization of members of the fibroblast growth factor family in the bovine mammary gland during different developmental and functional stages. Mammary tissue was obtained from German Brown Swiss cows(n = 23) during defined stages of mammogenesis (before and during pregnancy), lactogenesis, peak and late lactation, and involution. Extracted mRNA was analyzed by reverse transcription polymerase chain reaction and RNase protectionassay. The tissue content of fibroblast growth factor-1 and fibroblast growth factor-2 was determined by radio-immunoassay, and the localization of fibroblast growth factor-2 was determined by immunohistochemistry. The highest mRNA concentration for fibroblast growth factor-1, -2, and -7 and their receptors was detected in the glands of virgin heifers or primigravid heifers during involution; less abundant fibroblast growth factor mRNA was detected during lactogenesis and galactopoiesis. Tissue protein concentrations of fibroblast growth factor-1 and fibroblast growth factor-2 showed similar tendencies. Immunoreactive fibroblast growth factor-2 was observed during mammogenesis and involution in endothelial cells, ductal epithelial cells, myoepithelial cells, and some alveolar cells. After positive staining, fibroblast growth factor-2 could only be observed in endothelial and myoepithelial cells during lactogenesis and could only be observed in myoepithelial cells during galactopoiesis, Expression, tissue concentration, and distinct localization suggest that fibroblast growth factors may be important inthe local regulation of the bovine mammary gland.

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Documento generato il 04/12/20 alle ore 22:39:01