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Titolo:
EXAMINATION OF THE ROLE OF DNA-POLYMERASE PROOFREADING IN THE MUTATOREFFECT OF MISCODING TRANSFER-RNAS
Autore:
SLUPSKA MM; KING AG; LU LI; LIN RH; MAO EF; LACKEY CA; CHIANG JH; BAIKALOV C; MILLER JH;
Indirizzi:
UNIV CALIF LOS ANGELES,DEPT MICROBIOL & MOL GENET LOS ANGELES CA 90095 UNIV CALIF LOS ANGELES,DEPT MICROBIOL & MOL GENET LOS ANGELES CA 90095 UNIV CALIF LOS ANGELES,INST MOL BIOL LOS ANGELES CA 90095
Titolo Testata:
Journal of bacteriology (Print)
fascicolo: 21, volume: 180, anno: 1998,
pagine: 5712 - 5717
SICI:
0021-9193(1998)180:21<5712:EOTROD>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
NONPOLYPOSIS COLON-CANCER; EXONUCLEASE ACTIVE-SITE; ESCHERICHIA-COLI; 3'-5' EXONUCLEASE; MISMATCH REPAIR; III HOLOENZYME; GENE; HOMOLOG; MUTAGENESIS; MUTATIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
M.M. Slupska et al., "EXAMINATION OF THE ROLE OF DNA-POLYMERASE PROOFREADING IN THE MUTATOREFFECT OF MISCODING TRANSFER-RNAS", Journal of bacteriology (Print), 180(21), 1998, pp. 5712-5717

Abstract

We previously described Escherichia coli mutator tRNAs that insert glycine in place of aspartic acid and postulated that the elevated mutation rate results from generating a mutator polymerase. We suggested that the proofreading subunit of polymerase III, epsilon, is a likely target fur the aspartic acid-to-glycine change that leads to a lowered fidelity of replication, since the altered epsilon subunits resulting from this substitution (approximately 1% of the time) are sufficient tocreate a mutator effect, based on several observations of mutD alleles. In the present work, we extended the study of specific mutD allelesand constructed 16 altered mutD genes by replacing each aspartic acidcodon, in series, with a glycine codon in the dnaQ gene that encodes epsilon, We show that three of these genes confer a strong mutator effect. We have also looked for new mutator tRNAs and have found one: a glycine tRNA that inserts glycine at histidine codons. We then replacedeach of the seven histidine codons in the mutD gene with glycine codons and found that in two cases, a strong mutator phenotype results. These findings are consistent with the epsilon subunit playing a major role in the mutator effect of misreading tRNAs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 07:01:01