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Titolo:
THE INHIBITORY EFFECT OF AN EGF RECEPTOR-SPECIFIC TYROSINE KINASE INHIBITOR ON PANCREATIC-CANCER CELL-LINES WAS MORE POTENT THAN INHIBITORYANTIBODIES AGAINST THE RECEPTORS FOR EGF AND IGF-1
Autore:
KOBARI M; KULLENBERG B; BJORKMAN A; MATSUNO S; IHSE I; AXELSON J;
Indirizzi:
UNIV LUND HOSP,DEPT SURG S-22185 LUND SWEDEN UNIV LUND HOSP,DEPT SURG S-22185 LUND SWEDEN TOHOKU UNIV,DEPT SURG 1 SENDAI MIYAGI JAPAN TOHOKU UNIV,SCH MED SENDAI MIYAGI 980 JAPAN
Titolo Testata:
International journal of pancreatology
fascicolo: 2, volume: 24, anno: 1998,
pagine: 85 - 95
SICI:
0169-4197(1998)24:2<85:TIEOAE>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL GROWTH-FACTOR; FACTOR-I; FACTOR-ALPHA; NUDE-MICE; REGULATORY PEPTIDES; CARCINOMA INVITRO; A431 CELLS; EXPRESSION; AUTOCRINE; TYRPHOSTINS;
Keywords:
PANCREATIC CANCER; CELL LINES; CELL PROLIFERATION; EGF; IGF-1; TYRPHOSTIN; EGF RECEPTOR ANTIBODY; IGF-1 RECEPTOR ANTIBODY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
M. Kobari et al., "THE INHIBITORY EFFECT OF AN EGF RECEPTOR-SPECIFIC TYROSINE KINASE INHIBITOR ON PANCREATIC-CANCER CELL-LINES WAS MORE POTENT THAN INHIBITORYANTIBODIES AGAINST THE RECEPTORS FOR EGF AND IGF-1", International journal of pancreatology, 24(2), 1998, pp. 85-95

Abstract

Conclusion, Epidermal growth factor (EGF) increased the cell number of the two pancreatic cancer cell lines, MiaPaCa-2 and LN-36, in vitro,A blockade of the EGF-R tyrosine kinase with tyrphostin was more efficient in reducing the cell number than inhibiting receptor antibodies. IGF-1 increased the cell number, and blockade of the IGF-I-R initially decreased the cell number that later was followed by an increase in LN-36, Background/Aim. The receptors and ligands of EGF and insulin-like growth factor-1 (IGF-1) are overexpressed in pancreatic cancer tissue. The aim of the present experiments was to study the effects of EGFand IGF-1 on the cell number in two pancreatic cancer cell Lines. Material and Methods. MiaPaCa-2 cells were grown in 0.2% fetal calf serum(FCS) and the newly established LN-36 cells in serum-free medium (SFM). The cell number was measured with the XTT method. The effects of EGF and IGF-1 were studied in combination with inhibiting receptor antibodies and an EGFR-specific tyrosine kinase inhibitor, tyrphostin B56. Results, MiaPaCa-2 responded with increased cell number to stimulationwith EGF, and at 10(-8)M or higher concentrations a dose-response pattern was seen. Administration of B56 to MiaPaCa-2 decreased the cell number by 87%. The inhibiting EGF-R-Ab only inhibited EGF-induced increase in cell number. IGF-1 doubled the cell number of MiaPaCa-2 and increased the cell growth induced by EGF. The inhibiting IGF-1-R-Ab reduced the cell number by 10%. The LN-36 cell line responded to EGF with an increased cell number with a maximum at 5 x 10(-9) M after 96 h. B56reduced the cell number by 90% at 10(-5) M, With less effect during stimulation with EGF. In contrast to B56, the inhibiting EGF-R-Ab in the same experiment did not reduce the cell number. LN-36 responded to IGF-1 with an increased cell number, but EGF-stimulated growth was not influenced. The inhibiting IGF-1-R-Ab reduced the cell number and suppressed the IGF-1 stimulated increase after 24 h and later it induced an increased cell number.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 07:05:23