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Titolo:
A HISTOCHEMICAL EXAMINATION OF THE STAINING OF KAINATE-INDUCED NEURONAL DEGENERATION BY ANIONIC DYES
Autore:
KIERNAN JA; MACPHERSON CM; PRICE A; SUN T;
Indirizzi:
UNIV WESTERN ONTARIO,DEPT CELL BIOL & ANAT LONDON ON N6A 5C1 CANADA
Titolo Testata:
Biotechnic & histochemistry
fascicolo: 5, volume: 73, anno: 1998,
pagine: 244 - 254
SICI:
1052-0295(1998)73:5<244:AHEOTS>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIGHT MICROSCOPIC RESPONSE; CONCUSSIVE HEAD-INJURY; SINGLE-STRANDED RNA; KAINIC ACID; MAGNESIUM-CHLORIDE; GLOBAL-ISCHEMIA; CUPROLINIC BLUE; BRAIN-DAMAGE; DARK NEURONS; RAT;
Keywords:
ACID FUCHSINE; ACIDOPHILIA; DEGENERATION; NEURON; STAINING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
J.A. Kiernan et al., "A HISTOCHEMICAL EXAMINATION OF THE STAINING OF KAINATE-INDUCED NEURONAL DEGENERATION BY ANIONIC DYES", Biotechnic & histochemistry, 73(5), 1998, pp. 244-254

Abstract

Anionic dyes, notably acid fuchsine, strongly stain the nuclei and cytoplasm of neurons severely damaged by injury or disease. We provide detailed instructions for staining nervous tissue with toluidine blue and acid fuchsine for optimal demonstration of injured neurons, Degeneration was induced in the hippocampus of the mouse by systemic administration of kainic acid, and the resulting acidophilia was investigated using paraffin sections of the Carnoyor Bouin-fixed brains. The affected cells were bright red with the toluidine blue-acid fuchsine sequence. Their nuclei were stainable also with alkaline Biebrich scarlet andwith the 1,2-naphthoquinone-4-sulfonic acid-Ba[OH](2) method; all staining was blocked by benzil but was relatively refractory to deamination by HNO2, These properties indicated an arginine-rich protein, The nuclei were strongly acidophilic in the presence of a high concentration of DNA (strong Feulgen reaction), and acidophilia could not be induced in normal neuronal nuclei by chemical extraction of nucleic acids, The cytoplasmic acidophilia of degenerating hippocampal neurons was due to a protein rich in lysine (extinguished by alkalinity, easily prevented by deamination, and unaffected by benzil), Stainable RNA was absent from the perikarya of the affected cells, but normal neuronal cytoplasm did not become acidophilic after extraction of nucleic acids, Wesuggest that kainate-induced cell death is preceded by increased production of basic proteins, which become concentrated in the nucleus andperikaryon. Groups of small, darkly staining neurons were seen in thecerebral cortex in control and kainate-treated mice. These shrunken cells were purple with the toluidine blue-acid fuchsine stain, and wereattributed to local injury incurred during removal of the unfixed brain.

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Documento generato il 22/09/20 alle ore 09:45:30