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Titolo:
METABOLIC RECOVERY OF ISOLATED ADULT-RAT CARDIOMYOCYTES AFTER ENERGY DEPLETION - EXISTENCE OF AN ATP THRESHOLD
Autore:
BONZ A; SIEGMUND B; LADILOV Y; VAHL CF; PIPER HM;
Indirizzi:
UNIV GIESSEN,INST PHYSIOL,AULWEG 129 D-35292 GIESSEN GERMANY UNIV GIESSEN,INST PHYSIOL D-35292 GIESSEN GERMANY UNIV HEIDELBERG,CHIRURG KLIN,ABT HERZCHIRURG D-69120 HEIDELBERG GERMANY
Titolo Testata:
Journal of Molecular and Cellular Cardiology
fascicolo: 10, volume: 30, anno: 1998,
pagine: 2111 - 2119
SICI:
0022-2828(1998)30:10<2111:MROIAC>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
MYOCARDIAL ADENINE-NUCLEOTIDES; REOXYGENATED CARDIOMYOCYTES; 2,3-BUTANEDIONE MONOXIME; ISCHEMIC CONTRACTURE; MYOCYTES; INJURY; MUSCLE; ACIDOSIS; HEARTS; BDM;
Keywords:
ATP DEPLETION; METABOLIC INHIBITION; CARDIOMYOCYTES; IRREVERSIBLE CELL INJURY; ANOXIA/REOXYGENATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
A. Bonz et al., "METABOLIC RECOVERY OF ISOLATED ADULT-RAT CARDIOMYOCYTES AFTER ENERGY DEPLETION - EXISTENCE OF AN ATP THRESHOLD", Journal of Molecular and Cellular Cardiology, 30(10), 1998, pp. 2111-2119

Abstract

The question was investigated whether cardiomyocytes can be resuscitated after extreme energy depletion, i.e. after loss of ATP >70%. Isolated ventricular cardiomyocytes of the adult rat were exposed to metabolic inhibition with dinitrophenol and cyanide (DNP 0.2 mM: KCN 2 mM). After rapid energy depletion, cells were ''reoxygenated'' by wash-out of DNP and KCN. Intracellular calcium, cell length, ATP and creatine phosphate (CrP) of the cardiomyocytes were monitored. Metabolic inhibition resulted in a depletion of the stores of ATP and CrP by more than 95% of the normoxic values and caused a cytosolic Ca2+ overload. Parameters of metabolic recovery were: (i) resynthesis of CrP; (ii) recovery of a normal cytosolic Ca2+ control; and (iii) the elicitation of energy-dependent hypercontracture. ''Reoxygenation'', i.e. wash-out of metabolic inhibitors, reactivated oxidative phosphorylation. Consecutively, CrP levels recovered to 76.0 +/- 7.3%, ATP levels recovered to 10.4 +/- 2.3% (means +/- S.D., n = 10) of the initial normoxic values, a normoxic intracellular calcium level was re-established and hypercontracture was elicited. Prolongation of metabolic inhibition with KCN (2 mM) or inhibition of the Na+/K+ pump with ouabain (0.5 mM) disabled the cardiomyocytes to recover from cytosolic Ca2+ overload and preventedhypercontracture. It is concluded that even after extensive energy depletion metabolic resuscitation of the myocardial cell remains possible and a critical range of ATP for recovery i.e. a ''threshold'' of a 70% loss of ATP, does not exist. (C) 1998 Academic Press.

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Documento generato il 03/12/20 alle ore 15:41:45