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Titolo:
ADIPOPHILIN IS A SPECIFIC MARKER OF LIPID-ACCUMULATION IN DIVERSE CELL-TYPES AND DISEASES
Autore:
HEID HW; MOLL R; SCHWETLICK I; RACKWITZ HR; KEENAN TW;
Indirizzi:
GERMAN CANC RES CTR,DIV CELL BIOL,NEUENHEIMER FELD 280 D-69120 HEIDELBERG GERMANY UNIV HALLE WITTENBERG,INST PATHOL D-06097 HALLE GERMANY VIRGINIA POLYTECH INST & STATE UNIV,DEPT BIOCHEM BLACKSBURG VA 24061
Titolo Testata:
Cell and tissue research
fascicolo: 2, volume: 294, anno: 1998,
pagine: 309 - 321
SICI:
0302-766X(1998)294:2<309:AIASMO>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIFFERENTIATION-RELATED PROTEIN; MAMMARY EPITHELIAL-CELLS; INTERMEDIATE FILAMENTS; ENDOPLASMIC-RETICULUM; DESMOSOMAL PLAQUE; GLOBULE-MEMBRANE; ADRENAL-CELLS; EXPRESSION; DROPLETS; VIMENTIN;
Keywords:
ADIPOPHILIN; LIPID DROPLETS; FAT-ACCUMULATING CELLS; LIPID-BINDING PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
H.W. Heid et al., "ADIPOPHILIN IS A SPECIFIC MARKER OF LIPID-ACCUMULATION IN DIVERSE CELL-TYPES AND DISEASES", Cell and tissue research, 294(2), 1998, pp. 309-321

Abstract

We report the human DNA and protein sequence of adipophilin and its association with the surface of lipid droplets. The amino acid sequenceof human adipophilin has been determined by using cDNA clones from several tissues and confirmed by the reverse transcription/polymerase chain reaction method and Edman sequencing. The open reading frame of adipophilin encodes a polypeptide with a calculated molecular weight of 48.1 kDa and an isoelectric point of 6.72. By immunofluorescence and electron-microscopic localization with newly raised specific poly- and monoclonal antibodies, we show that this protein is not restricted to adipocytes as previously indicated by studies of the mouse homologous protein, adipose-differentiation-related protein. Adipophilin occurs in a wide range of cultured cell lines, including fibroblasts and endothelial and epithelial cells. In tissues, however, expression of adipophilin is restricted to certain cell types, such as lactating mammary epithelial cells, adrenal cortex cells, Sertoli and Leydig cells of themale reproductive system, and steatosis or fatty change hepatocytes in alcoholic liver cirrhosis. Our results reveal adipophilin as a possible new marker for the identification of specialized differentiated cells containing lipid droplets and for diseases associated with fat-accumulating cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 20:14:52