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Titolo:
CHARACTERIZATION OF GUANOXABENZ REDUCING ACTIVITY IN RAT-BRAIN
Autore:
DAMBROVA M; UHLEN S; WIKBERG JES;
Indirizzi:
BMC,PHARMACEUT PHARMACOL,BOX 591 S-75124 UPPSALA SWEDEN UPPSALA UNIV,DIV PHARMACOL,DEPT PHARMACEUT BIOSCI UPPSALA SWEDEN
Titolo Testata:
Pharmacology & toxicology
fascicolo: 4, volume: 83, anno: 1998,
pagine: 158 - 163
SICI:
0901-9928(1998)83:4<158:COGRAI>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
2 FORMS; GUANABENZ; MECHANISM; EXISTENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
M. Dambrova et al., "CHARACTERIZATION OF GUANOXABENZ REDUCING ACTIVITY IN RAT-BRAIN", Pharmacology & toxicology, 83(4), 1998, pp. 158-163

Abstract

2,6-dichlorobenzylidene-amino)-3-hydroxyguanidine) and enz(1-(2,6-dichlorobenzylidene-amino)-3-guanidine) are both known as centrally active antihypertensive drugs. We have previously shown that enzymatic activity in the rat spleen can induce N-reduction of guanoxabenz, leading to high affinity alpha(2)-adrenoceptor binding, due to the formation of the alpha(2)-adrenoceptor active drug, guanabenz. The spleen activity appears to reside in xanthine oxidase as it is activated by xanthineand blocked by allopurinol. We report that high affinity guanoxabenz binding is also induced in rat brain membranes after addition of NADH or NADPH cofactors. However, the brain process was clearly different from that of the spleen, as the formation of high affinity binding in the brain was not blocked by allopurinol. Moreover the NADH/NADPH activated mechanism of the brain membranes was not blocked by carbon monoxide and SKF525A, thus the activity appears not to reside in cytochrome P450 enzymes. Instead the activity was blocked by menadione and dicumarol. We conclude that the rat cerebral cortex contains an enzymatic activity that may activate guanoxabenz leading to formation of a metabolite showing high affinity for alpha 2-adrenoceptors. We also conclude that the rat brain activity is clearly distinct from that of the rat spleen.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 08:04:11