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Titolo:
COMPLETION OF THE TULA HANTAVIRUS GENOME SEQUENCE - PROPERTIES OF THEL-SEGMENT AND HETEROGENEITY FOUND IN THE 3' TERMINI OF S-GENOME AND L-GENOME RNAS
Autore:
KUKKONEN SKJ; VAHERI A; PLYUSNIN A;
Indirizzi:
UNIV HELSINKI,DEPT VIROL,HAARTMAN INST,POB 21 FIN-00014 HELSINKI FINLAND
Titolo Testata:
Journal of General Virology
, volume: 79, anno: 1998,
parte:, 11
pagine: 2615 - 2622
SICI:
0022-1317(1998)79:<2615:COTTHG>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
VIRUS MESSENGER-RNA; STRAIN HALLNAS B1; SIN-NOMBRE-VIRUS; NUCLEOTIDE-SEQUENCE; HANTAAN VIRUS; GENETIC-CHARACTERIZATION; PUUMALA-HANTAVIRUS; CODING CAPACITY; TRANSCRIPTION; INITIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
S.K.J. Kukkonen et al., "COMPLETION OF THE TULA HANTAVIRUS GENOME SEQUENCE - PROPERTIES OF THEL-SEGMENT AND HETEROGENEITY FOUND IN THE 3' TERMINI OF S-GENOME AND L-GENOME RNAS", Journal of General Virology, 79, 1998, pp. 2615-2622

Abstract

In this study the L segment and the 5' and 3' termini of the S, M andL segments of the prototype Tula hantavirus (TUL) were sequenced, thus completing the first determination of the genome sequence of a hantavirus that has not been linked to any human disease. The TUL L segmentcomprises 6541 nt with one ORF of 6459 nt in the antigenome sense. This ORF potentially encodes a 2153 aa protein with a predicted molecular mass of 247 kDa, The amino acid sequence includes all the motifs conserved in RNA-dependent RNA polymerases. The 5' termini of all three genome RNAs (vRNAs) had the expected sequences conserved in hantaviruses. The 3' termini of M vRNAs were also conserved. However, the 3' termini of S and L vRNAs were heterogeneous as most of the sequenced 3' termini had either deletions of 1 to 22 nt or an extra 1 to 3 nt. No increase in the level of heterogeneity was seen in vRNAs of virions collected 3, 6, 9 and 12 days postinfection, suggesting that the heterogeneity already exists at the early stages of infection. The S and L vRNAsfrom infected cells had more truncated 3' termini than vRNAs from pelleted virus. Heterogeneity of the 3' termini of genome RNAs could decrease the efficiency of antigenome and mRNA syntheses and contribute tothe slow growth observed for TUL and other hantaviruses in cell culture.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 03:20:52