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Titolo:
CLONING AND CHARACTERIZATION OF THE MOUSE HISTONE DEACETYLASE-2 GENE
Autore:
ZENG YY; TANG CM; YAO YL; YANG WM; SETO E;
Indirizzi:
UNIV S FLORIDA,H LEE MOFFITT CANC CTR & RES INST,MOL ONCOL PROGRAM,COLL MED TAMPA FL 33612 UNIV S FLORIDA,H LEE MOFFITT CANC CTR & RES INST,MOL ONCOL PROGRAM,COLL MED TAMPA FL 33612 UNIV S FLORIDA,H LEE MOFFITT CANC CTR & RES INST,COLL MED,DEPT BIOCHEM & MOL BIOL TAMPA FL 33612
Titolo Testata:
The Journal of biological chemistry
fascicolo: 44, volume: 273, anno: 1998,
pagine: 28921 - 28930
SICI:
0021-9258(1998)273:44<28921:CACOTM>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION FACTOR AP-2; FACTOR-INDUCIBLE GENE; BINDING PROTEIN; N-COR; REPRESSION; PROMOTER; COMPLEX; YEAST; MAX; MYC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
70
Recensione:
Indirizzi per estratti:
Citazione:
Y.Y. Zeng et al., "CLONING AND CHARACTERIZATION OF THE MOUSE HISTONE DEACETYLASE-2 GENE", The Journal of biological chemistry, 273(44), 1998, pp. 28921-28930

Abstract

Histone deacetylase-2 (HDAC2) is a component of a complex that mediates transcriptional repression in mammalian cells. A mouse HDAC2 cDNA was used to identify several recombinant clones containing the entire mouse HDAC2 gene. The mouse HDAC2 gene spans over 36 kilobase pairs andis composed of 14 exons (ranging from 58 to 362 nucleotides in length) and 13 introns (ranging from 75 base pairs to 19 kilobase pairs in length). Primer extension analysis with total RNA from NIH3T3 cells revealed a major transcriptional start site at 221 base pairs 5' of the ATG translational start codon. Upstream of the transcriptional start site, no canonical TATA box was found, but binding sites for several known transcription factors were identified. Transient transfection studies with 5' deletion mutants localized the promoter to no more than 76 base pairs upstream from the major transcriptional start site. Fluorescence in situ hybridization mapped mouse HDAC2 to chromosomal location10B1, which is in close proximity to the growth factor-inducible genefisp-12. Information concerning the genomic organization and promoterof HDAC2 will be useful in studies of the regulation of histone deacetylase activities, which in turn are important in studies of the regulation of transcriptional repression in mammalian cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 07:19:35